Western Blot (WB)
(Sample Data: Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.)
Rabbit MEK1/2 Polyclonal Antibody | anti-phospho-MEK1/2 antibody
Anti-phospho-MEK1/2 (Ser218 Ser222)
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Xenopus, Zebrafish
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
MEK1/2; Polyclonal Antibody; Anti-phospho-MEK1/2 (Ser218 Ser222); anti-phospho-MEK1/2 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Xenopus, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the MEK1/2 protein phosphorylated at serine 218 and serine 222
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-MEK1/2 antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.)
Western Blot (WB)
(Sample Data: Western Blot of NIH 3T3 cell lysates showing specific immunolabeling of the ~45kDa MEK 1/2 protein phosphorylated at Ser218 and Ser222. The cells were either serum starved (Control) or incubated in the presence of serum (Serum). Immunolabeling of an additional band at ~95kDa was also observed.)
Related Product Information for anti-phospho-MEK1/2 antibody
MEK1 (MAP Kinase Kinase, also known as MKK) is a member of the dual specificity protein kinase family. MEK1 lies upstream of MAPK/ERK and stimulates the enzymatic activity of MAPK/ERK upon a wide variety of extra-and intracellular signals. As an essential component of the MAPK/ERK signal transduction pathway, MEK1 is involved in many cellular processes such as proliferation, differentiation, transcriptional regulation and development (1). This pathway plays a key role in synaptic plasticity in the brain (2). Constitutive activation of MEK1 results in cellular transformation. Thus, MEK 1 represents a likely target for pharmacological intervention in proliferative diseases such as cancer (3). MEK1 can be phosphorylated by MAP kinase at Thr292 and Thr386.
Product Categories/Family for anti-phospho-MEK1/2 antibody
References
1. Seger, R. et al: The MAPK signaling cascade. FASEB J. 1995 Jun;9(9):726-35.
2. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
3. Sebolt-Leopold, J S. et al: Blockade of the MAP Kinase Pathway Suppresses growth of colon tumors in vivo. Nature Med. 1999 5: 810-816.
2. Adams, J P. et al: Molecular psychology: Roles for the ERK MAP kinase cascade in memory. Annu Rev Pharmacol Toxicol 2002 42:135-163.
3. Sebolt-Leopold, J S. et al: Blockade of the MAP Kinase Pathway Suppresses growth of colon tumors in vivo. Nature Med. 1999 5: 810-816.
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Product Notes
The phospho-MEK1/2 (Catalog #AAA516064) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-MEK1/2 (Ser218 Ser222) reacts with Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Xenopus, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's MEK1/2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the phospho-MEK1/2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MEK1/2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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