Western Blot (WB)
(Sample Data: Western blot of rat cortex lysate showing specific labeling of the ~15kDa alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).)
Rabbit SNCA Polyclonal Antibody | anti-phospho-SNCA antibody
Anti-phospho-SNCA (Ser129)
Reactivity
Human, Mouse, Bovine, Canine, non-Human Primate
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
SNCA; Polyclonal Antibody; Anti-phospho-SNCA (Ser129); anti-phospho-SNCA antibody
Host
Rabbit
Reactivity
Human, Mouse, Bovine, Canine, non-Human Primate
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the SNCA protein phosphorylated at serine 129
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-SNCA antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser129 of Rat SNCA conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of rat cortex lysate showing specific labeling of the ~15kDa alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).)
Western Blot (WB)
(Sample Data: Western blot of rat cortex lysate showing specific labeling of the ~15kDa alpha synuclein protein phosphorylated at Ser129. Immunolabeling is blocked by the phosphopeptide (peptide) used as antigen but not by the corresponding dephosphopeptide (not shown).)
Related Product Information for anti-phospho-SNCA antibody
Alpha-synuclein is expressed in the presynaptic terminal of the neuron where it is implicated in the formation of SNARE complexes. Aggregated alpha-synuclein is one of the major components found in the Lewy bodies, which are found in the brains of patients with Parkinson's disease (PD) and other neurodegenerative disorders (1). In fact, there is evidence to suggest that early onset Parkinson's disease may be caused by a duplication or triplication of one of the alpha synuclein genes (2). Alpha-synuclein is also found in the brains of patients with multiple system atrophy (MSA) and amyotrophic lateral sclerosis (ALS). Recent evidence suggests that phosphorylation of alpha-synuclein at Ser129 plays a role in the formation of inclusions in PD (3,4).
Product Categories/Family for anti-phospho-SNCA antibody
References
1. Okochi, M. et al: Constitutive phosphorylation of the Parkinson's disease associated alpha-synuclein. J Biol Chem 2000 Jan 7;275(1): 390-7.
2. Chartier-Harlin, M C. et al: Alpha-synuclein locus duplication as a cause of familial Parkinson's disease. Lancet 2004 364: 1167-1169.
3. Smith, W W. et al: Alpha-synuclein phosphorylation enhances eosinophilic cytoplasmic inclusion formation in SH-SY5Y Cells. J Neurosci 2005 June 8; 25(23):5544-5552.
4. Masliah, E. et al: Effects of alpha-synuclein immunization in a mouse model of Parkinson's disease. Neuron 2005 Jun 16;46(6):857-868.
2. Chartier-Harlin, M C. et al: Alpha-synuclein locus duplication as a cause of familial Parkinson's disease. Lancet 2004 364: 1167-1169.
3. Smith, W W. et al: Alpha-synuclein phosphorylation enhances eosinophilic cytoplasmic inclusion formation in SH-SY5Y Cells. J Neurosci 2005 June 8; 25(23):5544-5552.
4. Masliah, E. et al: Effects of alpha-synuclein immunization in a mouse model of Parkinson's disease. Neuron 2005 Jun 16;46(6):857-868.
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Product Notes
The phospho-SNCA (Catalog #AAA516219) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-SNCA (Ser129) reacts with Human, Mouse, Bovine, Canine, non-Human Primate and may cross-react with other species as described in the data sheet. AAA Biotech's SNCA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the phospho-SNCA for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNCA, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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