Western Blot (WB)
(Sample Data: Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74kDa ATF2 protein phosphorylated at Ser490 and Ser498.)
Rabbit anti-Human, Rat ATF2 Polyclonal Antibody | anti-phospho-ATF2 antibody
Anti-phospho-ATF2 (Ser490 Ser498)
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
ATF2; Polyclonal Antibody; Anti-phospho-ATF2 (Ser490 Ser498); anti-phospho-ATF2 antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the ATF2 protein phosphorylated at serine 490/498
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-ATF2 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000
IHC 1:1000
IHC 1:1000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser490/498 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74kDa ATF2 protein phosphorylated at Ser490 and Ser498.)
Western Blot (WB)
(Sample Data: Western blot of human melanoma cells incubated with varying doses of the radiomimetic drug NCS showing specific immunolabeling of the ~74kDa ATF2 protein phosphorylated at Ser490 and Ser498.)
Related Product Information for anti-phospho-ATF2 antibody
The activating transcription factor ATF2 (also called CRE-BP1) is a member of the ATF/CREB family of leucine zipper transcription factors, which binds both AP-1 and Cre DNA response element (1). ATF2 has two unique characteristics: it mediates adenovirus E1A-induced trans-activation and forms a heterodimer with c-Jun (2). ATF2 is a target of the JNK signal transduction pathway (3). ATF2 is phosphorylated by JNK on two closely spaced threonine residues located in the NH2-terminal activation domain. The replacement of these phosphorylation sites with alanine inhibited the transcriptional activity of ATF2. Furthermore, p38 MAPK can also phosphorylate ATF2.
Product Categories/Family for anti-phospho-ATF2 antibody
References
1. Maekawa, T. et al: Leucine zipper structure of the protein CRE-BP1 binding to the cyclic AMP response element in brain. EMBO J 1989 8:2023-2028.
2. Nomura, N. et al: Isolation and characterization of a novel member of the gene family encoding the cAMP response element binding protein CRE-BP1. J Biol Chem. 1993 Feb 25;268(6):4259-66.
3. Gupta, S. et al: Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science, 1995 Jan 20;267(5196):389-93.
2. Nomura, N. et al: Isolation and characterization of a novel member of the gene family encoding the cAMP response element binding protein CRE-BP1. J Biol Chem. 1993 Feb 25;268(6):4259-66.
3. Gupta, S. et al: Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science, 1995 Jan 20;267(5196):389-93.
Similar Products
Product Notes
The phospho-ATF2 (Catalog #AAA515814) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-ATF2 (Ser490 Ser498) reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ATF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000 IHC 1:1000. Researchers should empirically determine the suitability of the phospho-ATF2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ATF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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