Western Blot (WB)
(Sample Data: Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK1 protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-PLK1(Thr210) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Rabbit PLK1 Polyclonal Antibody | anti-phospho-PLK1 antibody
Anti-phospho-PLK1 (Thr210)
Reactivity
Human, Mouse, Rat, Bovine, Canine, non-Human Primate, Xenopus, Zebrafish
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
PLK1; Polyclonal Antibody; Anti-phospho-PLK1 (Thr210); anti-phospho-PLK1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Canine, non-Human Primate, Xenopus, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the PLK1 protein phosphorylated at threonine 201
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-PLK1 antibody
Western Blot (WB)
Application Notes
WB: 1:1,000
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr210 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK1 protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-PLK1(Thr210) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Western Blot (WB)
(Sample Data: Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK1 protein phosphorylated at Thr210 (control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-phospho-PLK1(Thr210) antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.)
Related Product Information for anti-phospho-PLK1 antibody
PLK1 is a member of the Polo-Like Kinase family of Ser/Thr kinases. PLK1 localizes to centrosomes or spindle pole bodies and undergoes subcellular relocation during the cell cycle. Deregulated activities of PLKs often result in defective centrosome duplication, maturation, and/or microtubule dynamics (1). PLKs also regulate the function of the Golgi complex. Deregulated expression of human PLK1 is strongly correlated with the development of many types of malignancies. Furthermore, ectopic expression of PLK1 dominant negative protein leads to rapid cell death (2). It has been proposed that PLK1 function is altered at different stages of mitosis through consecutive phosphorylation events at Ser137 and Thr210 (3).
Product Categories/Family for anti-phospho-PLK1 antibody
References
1. Nigg, EA. et al: Dynamic changes in nuclear architecture during mitosis: on the role of protein phosphorylation in spindle assembly and chromosome segregation. Exp Cell Res. 1996 Dec 15;229
2. Dai, W. et al: Polo-like kinases and the microtubule organization center: targets for cancer therapies. Prog Cell Cycle Res. 2003;5:327-34.
3. van de Weerdt B.C., van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH (2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Mol. Cell Biol. Mar; 25(5):2031-44, 2005.
2. Dai, W. et al: Polo-like kinases and the microtubule organization center: targets for cancer therapies. Prog Cell Cycle Res. 2003;5:327-34.
3. van de Weerdt B.C., van Vugt MA, Lindon C, Kauw JJ, Rozendaal MJ, Klompmaker R, Wolthuis RM, Medema RH (2005) Uncoupling anaphase-promoting complex/cyclosome activity from spindle assembly checkpoint control by deregulating polo-like kinase 1. Mol. Cell Biol. Mar; 25(5):2031-44, 2005.
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Product Notes
The phospho-PLK1 (Catalog #AAA516151) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-PLK1 (Thr210) reacts with Human, Mouse, Rat, Bovine, Canine, non-Human Primate, Xenopus, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's PLK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1,000. Researchers should empirically determine the suitability of the phospho-PLK1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PLK1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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