Western Blot (WB)
(Sample Data: Western blot of forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45kDa CREB1 phosphorylated at Ser133 (Left lane). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).)
Rabbit CREB1 Polyclonal Antibody | anti-phospho-CREB1 antibody
Anti-phospho-CREB1 (Ser133)
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Sheep, Xenopus, Zebrafish
Applications
Western Blot, Immunohistochemistry
Purity
Affinity Chromatography
Synonyms
CREB1; Polyclonal Antibody; Anti-phospho-CREB1 (Ser133); anti-phospho-CREB1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Sheep, Xenopus, Zebrafish
Clonality
Polyclonal
Isotype
Rabbit, IgG
Specificity
Recognizes the CREB1 protein phosphorylated at serine 133
Purity/Purification
Affinity Chromatography
Form/Format
100 ul in 10mM HEPES (pH7.5), 150mM NaCl, 100 ug per ml BSA and 50% glycerol.
Applicable Applications for anti-phospho-CREB1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
WB: 1:1,000
IHC 1:100
IHC 1:100
Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser383 conjugated to KLH
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Western blot of forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45kDa CREB1 phosphorylated at Ser133 (Left lane). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).)
Western Blot (WB)
(Sample Data: Western blot of forskolin stimulated rat hippocampal lysate showing specific immunolabeling of the ~45kDa CREB1 phosphorylated at Ser133 (Left lane). Phosphospecificity is shown in the right lane where the signal is completely eliminated by treatment with lambda phosphatase (lambda-Ptase, 1200 units for 30 min).)
Immunostaining
(Sample Data: Immunostaining of cultured rat hippocampal slices showing nuclear labeling of CREB phosphorylated at Ser133 in brown.)
Immunostaining
(Sample Data: Immunostaining of cultured rat hippocampal slices showing nuclear labeling of CREB phosphorylated at Ser133 in brown.)
Related Product Information for anti-phospho-CREB1 antibody
Members of the cAMP responsive element binding protein (CREB) family are critical mediators of gene expression in response to extracellular signals. Thus, they are essential regulators of adaptive behavior and long-term memory formation (1). A variety of protein kinases including protein kinase A (PKA), mitogen-activated protein kinases (MAPKs), and Ca2+/calmodulin dependent protein kinases (CAMKs) phosphorylate CRE1B at Ser133, which is required for CREB-mediated transcription (2,3). Activation of CREB1 has been implicated in the survival of mammalian cells. Mice that do not express CREB in the central nervous system display extensive apoptosis of postmitotic neurons during development (4). CREB can bind promoters containing CRE and activator protein 1 (AP-1) sites either as homo-and heterodimers to control gene expression.
Product Categories/Family for anti-phospho-CREB1 antibody
References
1. Valverde, O. et al: Modulation of anxiety-like behavior and morphine dependene in CREB-deficient mice. Neuropsychopharmacology. 2004 Jun;29(6):1122-33.
2. Johannessen, M. et al: What turns CREB on? Cellular Signalling 2004 16:1211-1227.
3. Kornhauser, J M. et al: CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 2002 34:221-233.
4. Mantamadiotis, T. et al: Disruption of CREB function in brain leads to neurodegeneration. Nat genet. 2002 May; 31(1): 47-54.
2. Johannessen, M. et al: What turns CREB on? Cellular Signalling 2004 16:1211-1227.
3. Kornhauser, J M. et al: CREB transcriptional activity in neurons is regulated by multiple, calcium-specific phosphorylation events. Neuron 2002 34:221-233.
4. Mantamadiotis, T. et al: Disruption of CREB function in brain leads to neurodegeneration. Nat genet. 2002 May; 31(1): 47-54.
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Product Notes
The phospho-CREB1 (Catalog #AAA515911) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-phospho-CREB1 (Ser133) reacts with Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Sheep, Xenopus, Zebrafish and may cross-react with other species as described in the data sheet. AAA Biotech's CREB1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). WB: 1:1,000 IHC 1:100. Researchers should empirically determine the suitability of the phospho-CREB1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREB1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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