Rabbit 14-3-3 beta Polyclonal Antibody | anti-phospho-14-3-3beta antibody

Anti-phospho-14-3-3 beta (Ser58)

Cat. Num. AAA516301

• Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Sheep, Xenopus, Zebrafish
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity Chromatography
• Synonyms: 14-3-3 beta; Polyclonal Antibody; Anti-phospho-14-3-3 beta (Ser58); anti-phospho-14-3-3beta antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat, Bovine, Canine, Chicken, non-Human Primate, Sheep, Xenopus, Zebrafish
• Clonality: Polyclonal
• Isotype: Rabbit, IgG
• Specificity: Recognizes the 14-3-3 beta protein phosphorylated at serine 58
• Purity/Purification: Affinity Chromatography
• Form/Format: 100 ul in 10mM HEPES (pH7.5), 150mMNaCl, 100 ug per ml BSA and 50% glycerol.

• Applicable Applications for anti-phospho-14-3-3beta antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:1,000; IHC 1:500
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser58 conjugated to KLH
• Preparation and Storage: Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.

Western Blot (WB)

(Sample Data: Western blot of rat brainstem lysate showing specific immuno-labeling of the ~29k 14-3-3 beta protein phosphorylated at Ser58 (Control). The immunolabeling is blocked by the phosphopeptide used as the antigen (Phos-pep) but not by the corresponding dephosphopeptide (not shown).)

Immunofluorescence (IF)

(Sample Data: Immunofluorescence of 21d mouse brain cryosections showing specific staining of the 14-3-3 beta protein when phosphorylated at Ser58 in green and DNA in blue.)

Related Product Information for anti-phospho-14-3-3beta antibody

14-3-3beta is a member of the highly conserved family of 14-3-3 proteins that appear to have multiple roles in cell signaling by binding to phosphoserine-containing proteins (1). 14-3-3 proteins are abundantly expressed in the brain and have been detected in the cerebrospinal fluid of patients with different types of neurological disorders (2). 14-3-3 proteins bind and regulate protein ligands that are typically phosphorylated on serine or threonine residues (3). 14-3-3 proteins affect a diverse array of cellular processes including the cell cycle and transcription, signal transduction and intracellular trafficking. 14-3-3 proteins function as a dimer. Dimerization of the 14-3-3 protein is regulated by site-specific serine phosphorylation (4).

Product Categories/Family for anti-phospho-14-3-3beta antibody

Phospho Specific Antibodies; AKT/PKB Pathway; Cancer; Cell Cycle; Cellular Stress; ERK/MAPK Pathway; Neurobiology; PKA/PKC Pathway; WNT Signaling

References

1. Yaffe, M B. et al: The structure basis for 14-3-3:phosphopeptide binding specificity. Cell 1997 91: 961-971.
2. Berg, D. et al.: 14-3-3 Proteins in the nervous system. Nat Rev Neurosci 2003 4:752-762.
3. Silhan, J. et al.: 14-3-3 Protein C-terminal stretch occupies ligand binding groove and is displaced by phosphopeptide binding. J Biol Chem 2004 279:49113-49119.
4. Woodcock, J M. et al: The dimeric versus monomeric status of 14-3-3 zeta is controlled by phosphorylation of Ser58 at the dimer interface. J Biol Chem 2003 278:36323-36327.