Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT5 (1:1000) using 5 ng of human recombinant STAT1beta, STAT2, STAT3, STAT4, STAT5 and STAT6.)
Rabbit STAT5 Polyclonal Antibody | anti-STAT5 antibody
Anti-STAT5
Reactivity
Western blot STAT5 from human cellsSTAT5 from other species may also be detectable.
Applications
Western Blot
Purity
Affinity Chromatography
Synonyms
STAT5; Polyclonal Antibody; Anti-STAT5; anti-STAT5 antibody
Host
Rabbit
Reactivity
Western blot STAT5 from human cells
STAT5 from other species may also be detectable.
STAT5 from other species may also be detectable.
Clonality
Polyclonal
Specificity
Recognizes the STAT5 protein
Purity/Purification
Affinity Chromatography
Form/Format
PBS pH7.4, 0.02% sodium azide, 50% glycerol
Applicable Applications for anti-STAT5 antibody
Western Blot (WB)
Application Notes
WB: 21752:22751 dilution
Immunogen
Synthetic peptide corresponding to amino acids 692-696 of human STAT5 (protein accession number P42229)
Preparation and Storage
Store at 4 degree C (add 0.1% NaN3) for several months, and at -20 degree C for longer periods. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For optimal performance, avoid repeated handling and multiple freeze/thaw cycles.
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT5 (1:1000) using 5 ng of human recombinant STAT1beta, STAT2, STAT3, STAT4, STAT5 and STAT6.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT5 (1:1000) using 5 ng of human recombinant STAT1beta, STAT2, STAT3, STAT4, STAT5 and STAT6.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT5 (1:1000) using 3 ug, 10 ug and 30 ug of K562 cell lysate.)
Western Blot (WB)
(Sample Data: Representative western blot with Anti-STAT5 (1:1000) using 3 ug, 10 ug and 30 ug of K562 cell lysate.)
Related Product Information for anti-STAT5 antibody
STAT5 (signal transducers and activators of transcription 5) is a member of the STAT family. It is phosphorylated by receptor associated protein kinases in response to cytokines and growth factors (1). The differentiation of T helper cells is regulated by members of the STAT family. In BCR-ABL-positive cells, STAT5 is constitutively activated by tyrosine phosphorylation. STAT5 activation results in upregulation of Bcl-XL and increased resistance to induction of apoptosis. STAT5 is involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6 betahydroxylase (2).
Product Categories/Family for anti-STAT5 antibody
References
1. Wang, D. et al: Naturally occurring dominant negative variants of Stat5. Molec. Cell Biol. 16: 6141-6148, 1996.
2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173-2178.
2. A Subramanian et al: STAT 5 and NF-Y are involved in expression and growth hormone-mediated sexually dimorphic regulation of cytochrome P450 3A10/lithocholic acid 6beta-hydroxylase. Nucleic Acids Res. 1998; 26(9): 2173-2178.
Similar Products
Product Notes
The STAT5 (Catalog #AAA516247) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-STAT5 reacts with Western blot STAT5 from human cells STAT5 from other species may also be detectable. and may cross-react with other species as described in the data sheet. AAA Biotech's STAT5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 21752:22751 dilution. Researchers should empirically determine the suitability of the STAT5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "STAT5, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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