Sheep anti-Human, Mouse HMGA1B Polyclonal Antibody | anti-Hmga1 antibody

HMGA1B (High mobility group A1, Isoform B, HMG-Y)

Cat. Num. AAA623006

• Gene Names: Hmga1; Hmgi; Hmgy; Hmgiy; Hmga1a; Hmga1b; AL023995
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Affinity Purified; Purified by immunoaffinity chromatography.
• Synonyms: HMGA1B; Polyclonal Antibody; HMGA1B (High mobility group A1; Isoform B; HMG-Y); Anti -HMGA1B (High mobility group A1; anti-Hmga1 antibody

• Host: Sheep
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMGA1B at ~20kD. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by immunoaffinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with sterile PBS, 0.02% sodium azide.

• Applicable Applications for anti-Hmga1 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: E. coli-derived recombinant human HMGA1B (aa1-96; Accession # P17096).
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

SDS-Page

(Detection of HMGA1B with MBS623006. 30ug of whole cell extracts from K562, 293T, HeLa and NIH3T3 cells were prepared, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with 1ug/ml sheep anti-HMGA1B antibody.)

Related Product Information for anti-Hmga1 antibody

HMGA1B (High mobility group A1, isoform B; also HMG-Y) is a 10-11kD member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences. It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well. Human HMGA1B is 96aa in length and contains three A-T hook DNA-binding domains (aa21-31; 42-52; 67-78). It is constitutively phosphorylated, and may undergo acetylation. There are three potential splice variants. All three show the same 11aa insertion after Pro34. In addition, one form shows an additional 56aa substitution for aa55-96, while a second shows a 14aa substitution for aa80-96. Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B.

Product Categories/Family for anti-Hmga1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 262331580
• NCBI GeneID: 15361
• NCBI Accession #: NP_001160009.1
• NCBI GenBank Nucleotide #: NM_001166537.1
• UniProt Accession #: P17095; Q91WV2; Q924L7; Q924L8
• Molecular Weight: 11,614 Da
• NCBI Official Full Name: high mobility group protein HMG-I/HMG-Y isoform b
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: Hmga1
• NCBI Official Synonym Symbols: Hmgi; Hmgy; Hmgiy; Hmga1a; Hmga1b; AL023995
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y; high mobility group protein I; high mobility group protein A1; high mobility group AT-hook protein 1
• UniProt Protein Name: High mobility group protein HMG-I/HMG-Y
• Protein Family: High mobility group protein
• UniProt Gene Name: Hmga1
• UniProt Synonym Gene Names: Hmgi; Hmgiy; HMG-I(Y)
• UniProt Entry Name: HMGA1_MOUSE

NCBI Description

This locus encodes a member of the nuclear, non-histone high mobility group protein family. This architectural transcription factor binds to A-T rich DNA sequences and participates in enhanceosome formation, chromatin remodeling and regulation of transcription. This protein functions in many cellular processes, including cell growth and differentiation. Alternatively spliced transcript variants have been described. [provided by RefSeq, Oct 2009]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Cellular Component: focal adhesion; nucleus

Molecular Function: retinoid X receptor binding; peroxisome proliferator activated receptor binding; enzyme binding; DNA-(apurinic or apyrimidinic site) lyase activity; ligand-dependent nuclear receptor transcription coactivator activity; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding

Biological Process: transcription from RNA polymerase II promoter; base-excision repair; spermatogenesis; positive regulation of transcription from RNA polymerase II promoter; DNA catabolic process, endonucleolytic