Testing Data
(Detection of NALP2: Lysates from human A549 and LNCaP cells were resolved by SDS-PAGE, transferred to Immobilon membranes and immunoblotted with 1ug/ml MBS631648.)
Mouse anti-Human NALP2 Monoclonal Antibody | anti-NLRP2 antibody
NALP2 (NACHT-, LRR- and PYD-Containing Protein 2, Nucleotide-binding Site Protein 1, PYRIN Domain and NACHT Domain-containing Protein 1, PYRIN-containing APAF1-like Protein 2, NLRP2, NBS1, PAN1, PYPAF2)
Gene Names
NLRP2; NBS1; PAN1; NALP2; PYPAF2; CLR19.9; FLJ20510
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
NALP2; Monoclonal Antibody; NALP2 (NACHT-; LRR- and PYD-Containing Protein 2; Nucleotide-binding Site Protein 1; PYRIN Domain and NACHT Domain-containing Protein 1; PYRIN-containing APAF1-like Protein 2; NLRP2; NBS1; PAN1; PYPAF2); Anti -NALP2 (NACHT-; anti-NLRP2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2A
Clone Number
11C41
Specificity
Recognizes human NALP2.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute in 100uL of PBS, 0.02% sodium azide.
Applicable Applications for anti-NLRP2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml
Dilution: Western Blot: 1ug/ml
Immunogen
Purified, E. coli-derived recombinant human NALP2 (rhNALP2; aa1-208; Accession # Q9NX02).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Testing Data
(Detection of NALP2: Lysates from human A549 and LNCaP cells were resolved by SDS-PAGE, transferred to Immobilon membranes and immunoblotted with 1ug/ml MBS631648.)
Testing Data
(Detection of NALP2: Lysates from human A549 and LNCaP cells were resolved by SDS-PAGE, transferred to Immobilon membranes and immunoblotted with 1ug/ml MBS631648.)
Related Product Information for anti-NLRP2 antibody
NALP2 (NAcht Leucine-rich repeat Protein 2, also known as PAN1) is a cytosolic, 121kD member of the NLRP family of proteins. It is expressed in macrophages and exhibits divergent effects on inflammation. Via NFkB, it suppresses TNF-alpha production in response to LPS; via procaspase-1, it promotes proIL-1-beta cleavage and release. Human NALP2 is 1062aa in length. It contains an N-terminal Pyrin domain (aa9-90), followed by a Nacht region and nine LRRs (aa467-1033). Four alternate isoforms exist. Three show deletions of aa109-132, 133-154 and 847-1062, respectively; a fourth shows a 38aa substitution for aa1-903. No close rodent counterparts have been reported.
Product Categories/Family for anti-NLRP2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
22,291 Da
NCBI Official Full Name
NACHT, LRR and PYD domains-containing protein 2 isoform 3
NCBI Official Synonym Full Names
NLR family, pyrin domain containing 2
NCBI Official Symbol
NLRP2
NCBI Official Synonym Symbols
NBS1; PAN1; NALP2; PYPAF2; CLR19.9; FLJ20510
NCBI Protein Information
NACHT, LRR and PYD domains-containing protein 2; OTTHUMP00000236775; OTTHUMP00000236776; OTTHUMP00000236777; OTTHUMP00000236779; OTTHUMP00000236780; nucleotide-binding site protein 1; PYRIN-containing APAF1-like protein 2; NACHT, LRR and PYD containing protein 2; NACHT, leucine rich repeat and PYD containing 2; PYRIN domain and NACHT domain-containing protein 1; nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domain containing 2
UniProt Protein Name
NACHT, leucine rich repeat and PYD containing 2, isoform CRA_c
Protein Family
UniProt Gene Name
PP1579
UniProt Synonym Gene Names
NALP2
UniProt Entry Name
Q8WY49_HUMAN
NCBI Description
NALP proteins, such as NALP2, are characterized by an N-terminal pyrin (MIM 608107) domain (PYD) and are involved in the activation of caspase-1 (CASP1; MIM 147678) by Toll-like receptors (see TLR4; MIM 603030). They may also be involved in protein complexes that activate proinflammatory caspases (Tschopp et al., 2003 [PubMed 12563287]).[supplied by OMIM]
Uniprot Description
NLRP2: Suppresses TNF- and CD40-induced NFKB1 activity at the level of the IKK complex, by inhibiting NFKBIA degradation induced by TNF. When associated with PYCARD, activates CASP1, leading to the secretion of mature proinflammatory cytokine IL1B. May be a component of the inflammasome, a protein complex which also includes PYCARD, CARD8 and CASP1 and whose function would be the activation of proinflammatory caspases. Belongs to the NLRP family. 4 isoforms of the human protein are produced by alternative splicing.
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: cytoplasm
Molecular Function: protein binding; Pyrin domain binding; ATP binding
Biological Process: positive regulation of caspase activity; positive regulation of interleukin-1 beta secretion
Research Articles on NLRP2
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Product Notes
The NLRP2 pp1579 (Catalog #AAA631648) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NALP2 (NACHT-, LRR- and PYD-Containing Protein 2, Nucleotide-binding Site Protein 1, PYRIN Domain and NACHT Domain-containing Protein 1, PYRIN-containing APAF1-like Protein 2, NLRP2, NBS1, PAN1, PYPAF2) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NALP2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the NLRP2 pp1579 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NALP2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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