Rabbit HXK III Polyclonal Antibody | anti-HXKIII antibody

HXK III (V848) Polyclonal Antibody

Cat. Num. AAA3002672

• Gene Names: HK3; HXK3; HKIII
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: HXK III; Polyclonal Antibody; HXK III (V848) Polyclonal Antibody; Hexokinase-3; Hexokinase3; Hexokinase 3; Hexokinase type III; HK III; HK3; anti-HXKIII antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: HXK III (V848) polyclonal antibody detects endogenous levels of HXK III protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 923

• Applicable Applications for anti-HXKIII antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 820-870 of Human HXK III.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of HXK III (V848) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:H1792 whole cell lysate (40ug) Lane3:AML-12 whole cell lysate (40ug) Lane4:PC12 whole cell lysate (40ug))

Related Product Information for anti-HXKIII antibody

Hexokinases phosphorylate glucose to produce glucose 6 phosphate, thus committing glucose to the glycolytic pathway. In vertebrates there are four major glucose phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase). Similar to hexokinases I and II, the allosteric enzyme hexokinase III is inhibited by its product glucose 6 phosphate. The catalytic activity of hexokinase III is associated with the C terminus while regulatory function is associated with the N terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 194097330
• NCBI GeneID: 3101
• NCBI Accession #: NP_002106.2
• NCBI GenBank Nucleotide #: NP_002106.2
• UniProt Accession #: P52790
• Molecular Weight: ~ 99kDa
• NCBI Official Full Name: hexokinase-3
• NCBI Official Synonym Full Names: hexokinase 3
• NCBI Official Symbol: HK3
• NCBI Official Synonym Symbols: HXK3; HKIII
• NCBI Protein Information: hexokinase-3
• UniProt Protein Name: Hexokinase-3
• UniProt Gene Name: HK3
• UniProt Synonym Gene Names: HK III
• UniProt Entry Name: HXK3_HUMAN

NCBI Description

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 3. Similar to hexokinases 1 and 2, this allosteric enzyme is inhibited by its product glucose-6-phosphate. [provided by RefSeq, Apr 2009]

Uniprot Description

HK3: a glycolytic enzyme that catalyzes the reaction ATP + D-hexose = ADP + D-hexose 6-phosphate. The first and rate-limiting step in glycosis, a pathway that produces energy in the form of ATP from glucose. An allosteric enzyme inhibited by its product glucose-6-phosphate (Glc-6-P). Acts as a ""glucose sensor"" by trapping glucose inside the cell by catalyzing its phosphorylation to produce Glc-6-P. In vertebrates there are four major glucose-phosphorylating isoenzymes, designated hexokinase I, II, III and IV (glucokinase).

Protein type: EC 2.7.1.1; Carbohydrate Metabolism - starch and sucrose; Kinase, other; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Carbohydrate Metabolism - galactose; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Transferase; Enzyme, cellular metabolism; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 5q35.2

Cellular Component: protein complex; mitochondrion; cytosol

Molecular Function: hexokinase activity; enzyme binding; mannokinase activity; hormone binding; fructokinase activity; glucokinase activity; ATP binding

Biological Process: hexose metabolic process; glycolysis; hexose transport; glucose 6-phosphate metabolic process; carbohydrate phosphorylation; carbohydrate metabolic process; cell glucose homeostasis; pathogenesis; glucose transport; transmembrane transport