SDS-Page
(Detection of Uromodulin. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml of MBS621352.)
Sheep anti-Mouse Tamm-Horsfall Glycoprotein Polyclonal Antibody
Tamm-Horsfall Glycoprotein (Uromucoid, THgp, Uromodulin, UMOD)
Reactivity
Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by affinity chromatography. Endotoxin: <0.1 EU/ug (LAL)
Synonyms
Tamm-Horsfall Glycoprotein; Polyclonal Antibody; Tamm-Horsfall Glycoprotein (Uromucoid; THgp; Uromodulin; UMOD); Anti -Tamm-Horsfall Glycoprotein (Uromucoid; anti-Tamm-Horsfall Glycoprotein antibody
Host
Sheep
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Tamm-Horsfall Glycoprotein. Shows <5% crossreactivity with recombinant human Tamm-Horsfall Glycoprotein.
Purity/Purification
Affinity Purified
Purified by affinity chromatography. Endotoxin: <0.1 EU/ug (LAL)
Purified by affinity chromatography. Endotoxin: <0.1 EU/ug (LAL)
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Applicable Applications for anti-Tamm-Horsfall Glycoprotein antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well.
Western Blot: 1ug/ml
Western Blot: 1ug/ml
Immunogen
NS0-derived, recombinant mouse Tamm-Horsfall Glycoprotein (aa24-618, Accession #Q91X17).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of Uromodulin. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml of MBS621352.)
SDS-Page
(Detection of Uromodulin. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml of MBS621352.)
Related Product Information for anti-Tamm-Horsfall Glycoprotein antibody
Tamm-Horsfall glycoprotein (THP, Uromodulin) is a 105-120kD urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL-1, TNF-alpha and C1q, activates resting monocytes, and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidney tubules. Mouse Uromodulin is GPI-linked. Its proprecursor is 619aa in length. It contains three EGF-like domains (aa28-148), a ZP domain that mediates oligomerization (aa335-590), and a cleavable C-terminal propeptide (aa619- 642). There are multiple splice variants. One shows an alternate start site at Met343, and there are two substitutions, a 17aa substitution for aa601-642, and a 166aa substitution for aa441- 642. Over aa25-614, mouse Uromodulin shares 78% and 89%aa identical to human and rat Uromodulin, respectively.
Product Categories/Family for anti-Tamm-Horsfall Glycoprotein antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Tamm-Horsfall protein - rat
Similar Products
Product Notes
The Tamm-Horsfall Glycoprotein (Catalog #AAA621352) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Tamm-Horsfall Glycoprotein (Uromucoid, THgp, Uromodulin, UMOD) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tamm-Horsfall Glycoprotein can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Dilution: Direct ELISA: 0.5-1.0ug/ml. The detection limit is ~0.3ng/well. Western Blot: 1ug/ml. Researchers should empirically determine the suitability of the Tamm-Horsfall Glycoprotein for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tamm-Horsfall Glycoprotein, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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