Goat anti-Human HMGA1 Polyclonal Antibody | anti-HMGA1 antibody

HMGA1 (AA9-21) (high mobility group AT-hook 1, HMG-R, HMGA1A, HMGIY, MGC12816, MGC4242, MGC4854, OTTHUMP00000016222, OTTHUMP00000016223, OTTHUMP00000016224, OTTHUMP00000039618, high-mobility group (nonhistone chromosomal) protein isoforms I and Y, nonhist

Cat. Num. AAA622576

• Gene Names: HMGA1; HMG-R; HMGIY; HMGA1A; MGC4242; MGC4854; MGC12816
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Affinity Purified; Purified by affinity chromatography.
• Synonyms: HMGA1; Polyclonal Antibody; HMGA1 (AA9-21) (high mobility group AT-hook 1; HMG-R; HMGA1A; HMGIY; MGC12816; MGC4242; MGC4854; OTTHUMP00000016222; OTTHUMP00000016223; OTTHUMP00000016224; OTTHUMP00000039618; high-mobility group (nonhistone chromosomal) protein isoforms I and Y; nonhist; Anti -HMGA1 (AA9-21) (high mobility group AT-hook 1; anti-HMGA1 antibody

• Host: Goat
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMGA1 (AA9-21). Sequence Homology: mouse, rat, canine, bovine, porcine.
• Purity/Purification: Affinity Purified; Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

• Applicable Applications for anti-HMGA1 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: ELISA: 1:6000
• Immunogen: Synthetic peptide corresponding to aa9-21, C-SQPLASKQEKDGT from near the N Terminus of human HMGA1 (NP_665906.1; NP_002122.1).
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Chemiluminescence

(MBS622576 (0.5ug/ml) staining of Human Pancreas lysate (35ug protein in RIPA buffer) with (B) and without (A) blocking with the immunizing peptide. Primary incubation was 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-HMGA1 antibody

HMGA1, an 11KDa basic, DNA-binding non-histone nuclear protein, is a key member of HMGA protein family. It consists of a highly conserved AT-hook DNA-binding domain that mediates binding to AT-rich sequences in the minor groove of chromosomal DNA. It functions as architectural chromatin-binding transcription factor altering the conformation of DNA by modulating nuclear protein-DNA complexes. It is involved in many cellular processes including growth regulation, viral induction of beta-IFN gene and regulation of inducible gene transcription. It also physically interacts with transcription factors such as AP-1, Sp-1 and CAAT/enhancer binding protein-beta to modulate their ability to activate gene expression. It functions as a direct transcriptional target of c-Jun and c-Myc and is maximally expressed during embryonic development. Pathological role of HMGA1 include integration of retroviruses into chromosomes and enhancing metastasis of tumor cells.

Product Categories/Family for anti-HMGA1 antibody

Antibodies; Abs to Binding Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 55958733
• NCBI GeneID: 3159
• UniProt Accession #: Q5T6U8; P17096; Q6IPL9; Q6ZP45
• Molecular Weight: 10,679 Da
• NCBI Official Full Name: high mobility group AT-hook 1
• NCBI Official Synonym Full Names: high mobility group AT-hook 1
• NCBI Official Symbol: HMGA1
• NCBI Official Synonym Symbols: HMG-R; HMGIY; HMGA1A; MGC4242; MGC4854; MGC12816
• NCBI Protein Information: high mobility group protein HMG-I/HMG-Y; HMG-I(Y); OTTHUMP00000016222; OTTHUMP00000016223; OTTHUMP00000016224; high mobility group protein R; high mobility group protein A1; nonhistone chromosomal high-mobility group protein HMG-I/HMG-Y; high-mobility group (nonhistone chromosomal) protein isoforms I and Y
• UniProt Protein Name: High mobility group AT-hook 1
• Protein Family: High mobility group protein
• UniProt Gene Name: HMGA1
• UniProt Entry Name: Q5T6U8_HUMAN

NCBI Description

This gene encodes a non-histone protein involved in many cellular processes, including regulation of inducible gene transcription, integration of retroviruses into chromosomes, and the metastatic progression of cancer cells. The encoded protein preferentially binds to the minor groove of A+T-rich regions in double-stranded DNA. It has little secondary structure in solution but assumes distinct conformations when bound to substrates such as DNA or other proteins. The encoded protein is frequently acetylated and is found in the nucleus. At least seven transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Uniprot Description

HMGA1: HMG-I/Y bind preferentially to the minor groove of A+T rich regions in double stranded DNA. It is suggested that these proteins could function in nucleosome phasing and in the 3'-end processing of mRNA transcripts. They are also involved in the transcription regulation of genes containing, or in close proximity to A+T-rich regions. A chromosomal aberration involving HMGA1 is found in pulmonary chondroid hamartoma. Translocation t(6;14)(p21;q23-24) with RAD51B. Belongs to the HMGA family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding

Chromosomal Location of Human Ortholog: 6p21

Cellular Component: nucleoplasm; transcription factor complex; focal adhesion; nucleus; cytosol

Molecular Function: retinoid X receptor binding; peroxisome proliferator activated receptor binding; protein binding; DNA-(apurinic or apyrimidinic site) lyase activity; enzyme binding; DNA binding; ligand-dependent nuclear receptor transcription coactivator activity; AT DNA binding; retinoic acid receptor binding; 5'-deoxyribose-5-phosphate lyase activity; transcription factor binding

Biological Process: DNA unwinding during replication; negative regulation of cell proliferation; negative regulation of chromatin silencing; regulation of transcription, DNA-dependent; nucleosome disassembly; viral reproduction; transcription, DNA-dependent; base-excision repair; positive regulation of transcription, DNA-dependent; response to virus; protein complex assembly; negative regulation of transcription, DNA-dependent; DNA catabolic process, endonucleolytic