Human bone morphogenetic protein 3 ELISA Kit | BMP3 elisa kit

Human Bone morphogenetic protein 3, BMP3 ELISA Kit

Cat. Num. AAA943749

• Gene Names: BMP3; BMP-3A
• Reactivity: Human
• Synonyms: bone morphogenetic protein 3; Human Bone morphogenetic protein 3; BMP3 ELISA Kit; Human Bone morphogenetic protein 3 (BMP3) ELISA kit; BMP-3A; bone morphogenetic protein 3 (osteogenic) ; bone morphogenetic protein-3; osteogenin; BMP3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human BMP3. No significant cross-reactivity or interference between human BMP3 and analogues was observed.
• Sequence Length: 472

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6 pg/ml-1000 pg/ml
• Sensitivity: < 3.9 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BMP3 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BMP3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BMP3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BMP3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BMP3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 126507087
• NCBI GeneID: 651
• NCBI Accession #: NP_001192.2
• NCBI GenBank Nucleotide #: NM_001201.2
• UniProt Accession #: P12645; Q4VAS5
• Molecular Weight: 53,372 Da
• NCBI Official Full Name: bone morphogenetic protein 3 preproprotein
• NCBI Official Synonym Full Names: bone morphogenetic protein 3
• NCBI Official Symbol: BMP3
• NCBI Official Synonym Symbols: BMP-3A
• NCBI Protein Information: bone morphogenetic protein 3; BMP-3; osteogenin; bone morphogenetic protein-3; bone morphogenetic protein 3A; bone morphogenetic protein 3 (osteogenic)
• UniProt Protein Name: Bone morphogenetic protein 3
• Protein Family: Bone morphogenetic protein
• UniProt Gene Name: BMP3
• UniProt Synonym Gene Names: BMP3A; BMP-3; BMP-3A
• UniProt Entry Name: BMP3_HUMAN

NCBI Description

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein, also known as osteogenin, induces bone formation. [provided by RefSeq, Jul 2008]

Uniprot Description

BMP3: Negatively regulates bone density. Antagonizes the ability of certain osteogenic BMPs to induce osteoprogenitor differentitation and ossification. Belongs to the TGF-beta family.

Protein type: Secreted, signal peptide; Cytokine; Secreted

Chromosomal Location of Human Ortholog: 4q21

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; transforming growth factor beta receptor binding; receptor binding

Biological Process: regulation of apoptosis; ossification; cell-cell signaling; cartilage development; regulation of MAPKKK cascade; positive regulation of transcription from RNA polymerase II promoter; skeletal development; cell development; growth