SDS-Page
(30ug of whole cell extracts from exponentially growing MCF7, HepG2, HeLa, and Hek293 cells were prepared, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with MBS623132 (0.1ug/m), as described in and incubate the membrane for 1 hour at room temperature in Blocking Solution.)
Sheep anti-Human PIAS2 Polyclonal Antibody | anti-PIAS2 antibody
PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-ALPH
Gene Names
PIAS2; miz; MIZ1; SIZ2; PIASX; ZMIZ4; MGC102682; PIASX-BETA; PIASX-ALPHA
Reactivity
Human
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
PIAS2; Polyclonal Antibody; PIAS2 (Protein inhibitor of activated STAT; 2 Androgen; receptor-interacting protein 3; ARIP3; DAB2-interacting protein; DIP; E3 SUMO-protein ligase PIAS2; MGC102682; miz; Miz1; MIZ1; Msx-interacting zinc finger protein; PIAS-NY protein; PIASX; PIASX-ALPH; Anti -PIAS2 (Protein inhibitor of activated STAT; PIASX-AL; anti-PIAS2 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Detects endogenous human PIAS2 in Western blot with an approximate molecular weight of 63-70kD.
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS, 0.02% sodium azide.
Applicable Applications for anti-PIAS2 antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 0.1ug/ml
Dilution: Western Blot: 0.1ug/ml
Immunogen
Purified, E. coli-derived recombinant human PIAS2 aa 1-550; Accession# O75928.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(30ug of whole cell extracts from exponentially growing MCF7, HepG2, HeLa, and Hek293 cells were prepared, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with MBS623132 (0.1ug/m), as described in and incubate the membrane for 1 hour at room temperature in Blocking Solution.)
SDS-Page
(30ug of whole cell extracts from exponentially growing MCF7, HepG2, HeLa, and Hek293 cells were prepared, resolved by SDS-PAGE, and transferred to a PVDF membrane. The membrane was immunoblotted with MBS623132 (0.1ug/m), as described in and incubate the membrane for 1 hour at room temperature in Blocking Solution.)
Related Product Information for anti-PIAS2 antibody
PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx-) is a 45-70kD member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK-1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa11-45), a zinc-finger region (aa331-408), a SUMO-binding motif (aa467-472) and an NLS (aa484-492). There are multiple splice variants. PIAS2x- /ARIP3 shows a 22 aa substitution for aa 551-621, while PIAS-NY shows a 12 aa substitution for aa 1-8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504-621, and an alternate start site at Met10. Over aa 1-550, human PIAS2 shares 98% aa identity with mouse PIAS2.
Product Categories/Family for anti-PIAS2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
56,391 Da
NCBI Official Full Name
PIAS2 protein
NCBI Official Synonym Full Names
protein inhibitor of activated STAT, 2
NCBI Official Symbol
PIAS2
NCBI Official Synonym Symbols
miz; MIZ1; SIZ2; PIASX; ZMIZ4; MGC102682; PIASX-BETA; PIASX-ALPHA
NCBI Protein Information
E3 SUMO-protein ligase PIAS2; DIP; ARIP3; PIAS-NY protein; DAB2-interacting protein; Msx-interacting-zinc finger; zinc finger, MIZ-type containing 4; msx-interacting zinc finger protein; protein inhibitor of activated STAT x; androgen receptor-interacting protein 3
UniProt Protein Name
PIAS2 protein
Protein Family
UniProt Gene Name
PIAS2
UniProt Entry Name
Q2TA77_HUMAN
NCBI Description
This gene encodes a protein involved in the regulation of transcription factors involved in MAP kinase signaling. The symbol MIZ1 has also been associated with ZBTB17 which is a different gene located on chromosome 1. Two alternatively spliced transcripts encoding different isoforms have been described. [provided by RefSeq]
Uniprot Description
Sequence similarities: Contains 1 SAP domain. SAAS SAAS003034
Research Articles on PIAS2
Similar Products
Product Notes
The PIAS2 pias2 (Catalog #AAA623132) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-ALPH reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's PIAS2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 0.1ug/ml. Researchers should empirically determine the suitability of the PIAS2 pias2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PIAS2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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