Rabbit anti-Human TRAF4 Polyclonal Antibody | anti-TRAF4 antibody

TRAF4 (TNF Receptor Associated Factor 4, TNF Receptor-associated Factor 4, TRAF 4, TRAF-4, Cysteine-rich Domain Associated with RING and Traf Domains Protein 1, CART1, Metastatic Lymph Node Gene 62 Protein, MLN 62, MLN62, RING Finger Protein 83, RNF83) (B

Cat. Num. AAA6397041

• Gene Names: TRAF4; CART1; MLN62; RNF83
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: TRAF4; Polyclonal Antibody; TRAF4 (TNF Receptor Associated Factor 4; TNF Receptor-associated Factor 4; TRAF 4; TRAF-4; Cysteine-rich Domain Associated with RING and Traf Domains Protein 1; CART1; Metastatic Lymph Node Gene 62 Protein; MLN 62; MLN62; RING Finger Protein 83; RNF83) (B; anti-TRAF4 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRAF4.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
• Sequence Length: 2894

• Applicable Applications for anti-TRAF4 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human TRAF4, aa1-470 (NP_004286.2).
• Immunogen Sequence: MPGFDYKFLEKPKRRLLCPLCGKPMREPVQVSTCGHRFCDTCLQEFLSEGVFKCPEDQLPLDYAKIYPDPELEVQVLGLPIRCIHSEEGCRWSGPLRHLQGHLNTCSFNVIPCPNRCPMKLSRRDLPAHLQHDCPKRRLKCEFCGCDFSGEAYESHEGMCPQESVYCENKCGARMMRRLLAQHATSECPKRTQPCTYCTKEFVFDTIQSHQYQCPRLPVACPNQCGVGTVAREDLPGHLKDSCNTALVLCPFKDSGCKHRCPKLAMARHVEESVKPHLAMMCALVSRQRQELQELRRELEELSVGSDGVLIWKIGSYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSAFLNGNGSGEGTHLSLYIRVLPGAFDNLLEWPFARRVTFSLLDQSDPGLAKPQHVTETFHPDPNWKNFQKPGTWRGSLDESSLGFGYPKFISHQDIRKRNYVRDDAVFIRAAVELPRKILS
• Conjugate: Biotin
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of TRAF4 expression in transfected 293T cell line by TRAF4 polyclonal antibody. Lane 1: TRAF4 transfected lysate (53.5kD). Lane 2: Non-transfected lysate.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between TRAF4 and TRAF6 HeLa cells were stained with TRAF4 rabbit purified polyclonal 1:1200 and TRAF6 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Product Categories/Family for anti-TRAF4 antibody

Antibodies; TRAF

NCBI and Uniprot Product Information

• NCBI GI #: 1519242051
• NCBI GeneID: 9618
• NCBI Accession #: NM_004295.4
• NCBI GenBank Nucleotide #: NM_004295
• UniProt Accession #: Q9BUZ4
• NCBI Official Full Name: Homo sapiens TNF receptor associated factor 4 (TRAF4), mRNA
• NCBI Official Synonym Full Names: TNF receptor associated factor 4
• NCBI Official Symbol: TRAF4
• NCBI Official Synonym Symbols: CART1; MLN62; RNF83
• NCBI Protein Information: TNF receptor-associated factor 4
• UniProt Protein Name: TNF receptor-associated factor 4
• Protein Family: TNF receptor-associated factor
• UniProt Gene Name: TRAF4
• UniProt Synonym Gene Names: CART1; MLN62; RNF83; MLN 62
• UniProt Entry Name: TRAF4_HUMAN

NCBI Description

This gene encodes a member of the TNF receptor associated factor (TRAF) family. TRAF proteins are associated with, and mediate signal transduction from members of the TNF receptor superfamily. The encoded protein has been shown to interact with neurotrophin receptor, p75 (NTR/NTSR1), and negatively regulate NTR induced cell death and NF-kappa B activation. This protein has been found to bind to p47phox, a cytosolic regulatory factor included in a multi-protein complex known as NAD(P)H oxidase. This protein thus, is thought to be involved in the oxidative activation of MAPK8/JNK. Alternatively spliced transcript variants have been observed but the full-length nature of only one has been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

TRAF4: Adapter protein and signal transducer that links members of the tumor necrosis factor receptor (TNFR) family to different signaling pathways. Plays a role in the activation of NF-kappa-B and JNK, and in the regulation of cell survival and apoptosis. Regulates activation of NF-kappa-B in response to signaling through Toll-like receptors. Required for normal skeleton development, and for normal development of the respiratory tract. Required for activation of RPS6KB1 in response to TNF signaling. Modulates TRAF6 functions. Belongs to the TNF receptor-associated factor family. B subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; Adaptor/scaffold

Chromosomal Location of Human Ortholog: 17q11-q12

Cellular Component: tight junction; cytoskeleton; perinuclear region of cytoplasm; cytoplasm; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding; zinc ion binding; thioesterase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; WW domain binding; tumor necrosis factor receptor binding; protein kinase binding

Biological Process: regulation of apoptosis; apoptosis; positive regulation of protein kinase activity; respiratory tube development; activation of NF-kappaB-inducing kinase; positive regulation of JNK cascade; protein ubiquitination; respiratory gaseous exchange; signal transduction