Human Prostaglandin D2 Synthase, Brain ELISA Kit | PGD2S elisa kit

Human Prostaglandin D2 Synthase, Brain ELISA Kit

Cat. Num. AAA727083

• Gene Names: PTGDS; PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• Reactivity: Human
• Synonyms: Prostaglandin D2 Synthase; Brain; Human Prostaglandin D2 Synthase; Brain ELISA Kit; PGD2S elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 50-1000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This PGD2S ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human PGD2S. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PGD2S elisa kit

Principle of the Assay: PGD2S ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGD2S antibody and an PGD2S-HRP conjugate. The assay sample and buffer are incubated together with PGD2S-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGD2S concentration since PGD2S from samples and PGD2S-HRP conjugate compete for the anti-PGD2S antibody binding site. Since the number of sites is limited, as more sites are occupied by PGD2S from the sample, fewer sites are left to bind PGD2S-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGD2S concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 32171249
• NCBI GeneID: 5730
• NCBI Accession #: NP_000945.3
• NCBI GenBank Nucleotide #: NM_000954.5
• Molecular Weight: 21,029 Da
• NCBI Official Full Name: prostaglandin-H2 D-isomerase
• NCBI Official Synonym Full Names: prostaglandin D2 synthase 21kDa (brain)
• NCBI Official Symbol: PTGDS
• NCBI Official Synonym Symbols: PDS; PGD2; PGDS; LPGDS; PGDS2; L-PGDS
• NCBI Protein Information: prostaglandin-H2 D-isomerase; cerebrin-28; PGD2 synthase; beta-trace protein; prostaglandin D synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin D synthase; lipocalin-type prostaglandin-D synthase
• UniProt Protein Name: Prostaglandin-H2 D-isomerase
• UniProt Gene Name: PTGDS
• UniProt Synonym Gene Names: PDS; PGD2 synthase; PGDS; PGDS2
• UniProt Entry Name: PTGDS_HUMAN

NCBI Description

The protein encoded by this gene is a glutathione-independent prostaglandin D synthase that catalyzes the conversion of prostaglandin H2 (PGH2) to postaglandin D2 (PGD2). PGD2 functions as a neuromodulator as well as a trophic factor in the central nervous system. PGD2 is also involved in smooth muscle contraction/relaxation and is a potent inhibitor of platelet aggregation. This gene is preferentially expressed in brain. Studies with transgenic mice overexpressing this gene suggest that this gene may be also involved in the regulation of non-rapid eye movement sleep. [provided by RefSeq, Jul 2008]

Uniprot Description

PTGDS: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system. Belongs to the calycin superfamily. Lipocalin family.

Protein type: Lipid Metabolism - arachidonic acid; Endoplasmic reticulum; EC 5.3.99.2; Isomerase; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 9q34.2-q34.3

Cellular Component: Golgi apparatus; extracellular space; endoplasmic reticulum membrane; nuclear membrane; rough endoplasmic reticulum; perinuclear region of cytoplasm; extracellular region

Molecular Function: retinoid binding; prostaglandin-D synthase activity; transporter activity; fatty acid binding

Biological Process: transport; response to glucocorticoid stimulus; cyclooxygenase pathway; arachidonic acid metabolic process; prostaglandin biosynthetic process; regulation of circadian sleep/wake cycle, sleep