SDS-Page
(Detection of mZIP4H: Lysates from human 293T, and human 293T cells transfected with hZIP4H and mZIP4H were resolved by SDS-PAGE, transferred to an PVDF membrane, and immunoblotted with 1.0 μg/mL anti-ZIP4H.)
Goat anti-Mouse Zip4H Polyclonal Antibody
Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3)
Reactivity
Mouse
Applications
Western Blot
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Zip4H; Polyclonal Antibody; Zip4H (Tex11; Testis Expressed 11; Testis Expressed Sequence 11; TGC1; TSGA3); Anti -Zip4H (Tex11; anti-Zip4H antibody
Host
Goat
Reactivity
Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse ZIP4H. Species sequence homology: human (44%) and rat (73%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with PBS, 0.02% sodium azide.
Applicable Applications for anti-Zip4H antibody
Western Blot (WB)
Application Notes
Suitable for use in Western Blot.
Dilution: Western Blot: 1ug/ml Detects ZIP4H protein from 293T cells transfected with the mouse ZIP4H gene with an approximate molecular weight of 110kD.
Dilution: Western Blot: 1ug/ml Detects ZIP4H protein from 293T cells transfected with the mouse ZIP4H gene with an approximate molecular weight of 110kD.
Immunogen
E.coli-derived recombinant mouse ZIP4H (rmZIP4H; aa768-947; Accession # Q14AT2).
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS, 0.02% sodium azide. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
SDS-Page
(Detection of mZIP4H: Lysates from human 293T, and human 293T cells transfected with hZIP4H and mZIP4H were resolved by SDS-PAGE, transferred to an PVDF membrane, and immunoblotted with 1.0 μg/mL anti-ZIP4H.)
SDS-Page
(Detection of mZIP4H: Lysates from human 293T, and human 293T cells transfected with hZIP4H and mZIP4H were resolved by SDS-PAGE, transferred to an PVDF membrane, and immunoblotted with 1.0 μg/mL anti-ZIP4H.)
Related Product Information for anti-Zip4H antibody
Testis expressed sequence 11 (Tex11), also known as ZIP4 homolog, is a 947 aa X chromosome-encoded protein that is selectively expressed in primordial male germ cells. It associates with Nbs1 of the Mre11 protein complex which results in localization to areas of synaptonemal contact between meiotic chromosomes. During meiosis, ZIP4H/Tex11 promotes sister chromatid synapsis and crossover in response to programmed double stranded DNA breaks. ZIP4H/Tex11 deficiency leads to increased spermatocyte apoptosis and male sterility. Alternate splicing in mouse generates an additional isoform with a substitution (aa691-707) and truncation (aa708-947) at the C-terminus. Within aa768-947, mouse ZIP4H/Tex11 shares 44% and 73% aa sequence identity with human and rat ZIP4H/Tex11, respectively.
Product Categories/Family for anti-Zip4H antibody
Similar Products
Product Notes
The Zip4H (Catalog #AAA626530) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Zip4H (Tex11, Testis Expressed 11, Testis Expressed Sequence 11, TGC1, TSGA3) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Zip4H can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Suitable for use in Western Blot. Dilution: Western Blot: 1ug/ml Detects ZIP4H protein from 293T cells transfected with the mouse ZIP4H gene with an approximate molecular weight of 110kD. Researchers should empirically determine the suitability of the Zip4H for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Zip4H, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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