Western Blot (WB)
(Western Blot using anti-HVEM antibody HMHV-1B18 (MBS488601) Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 () at 0.01ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Rabbit anti-Mouse HVEM Monoclonal Antibody | anti-HVEM antibody
Anti-HVEM [HMHV-1B18]
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunoprecipitation, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
HVEM; Monoclonal Antibody; Anti-HVEM [HMHV-1B18]; CD270; TNF Receptor-like 2; ATAR; Herpes virus Entry Mediator A; HVEA; Herpes virus Entry Mediator; LIGHTR; Tumor Necrosis Factor Receptor-Like Gene2; Tumor Necrosis Factor Receptor-Like 2; TR2; Tumor Necrosis Factor Receptor Superfamily Member 14; TNFRSF14; CD40-Like Protein; anti-HVEM antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, kappa
Clone Number
HMHV-1B18
Specificity
This antibody is specific for Herpes Virus Entry Mediator (HVEM, TR2), a type I transmembrane protein of TNF-receptor superfamily. This receptor, which is expressed on most cell types, including T cells, B cells, monocytes, neutrophils, and dendritic cells. Binding of HSV viral envelope glycoprotein D (gD) to this receptor protein has been shown to be part of the viral entry mechanism. The cytoplasmic region of HVEM was found to bind to several TRAF family members, which may mediate the signal transduction pathways that activate the immune response. HVEM has also been demonstrated to be a unique ligand for BTLA (B and T lymphocyte attenuator). The conservation of the BTLA-HVEM interaction between mouse and human suggests that this system is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-HVEM antibody
Flow Cytometry (FC/FACS), Western Blot (WB), Immunoprecipitation (IP), Blocking (BL), Functional Assay (FA)
Application Notes
This antibody has been used in FACS to demonstrate that lymphatic endothelial cells mediate deletion only via programmed cell death-1 (PD-1) ligand 1 (Tewalt et al 2012) and in Western Blot to study the role of LIGHT in the pathogenesis of hepatitis (Anand et al 2006). This antibody has been also been used in vivo experiments to study the mechanisms by which TNFSF14 functions to promote airway remodelling in asthma (Sibilano et al 2016), to confirm that costimulatory role through HVEM is not necessary for LIGHT-mediated liver inflammation (Anand et at 2006), and to investigate the role that herpesvirus entry mediator plays in the development of experimental conjunctivitis (Ishida et al, 2012). Treatment with this antibody has been observed to diminish plasma levels of antigen-specific IgG1 and IgE antibodies in mouse asthma models (Sibilano et al 2016), to interfere with the LIGHT-HVEM interaction but not interaction between B and T lymphocyte attenuator (BTLA) and HVEM in mouse hepatitis models (Anand et at 2006), and NOT to affect the development of experimental conjunctivitis in either the induction or the effector phase (Ishida et al, 2012).
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
PMID
21779950
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Western Blot (WB)
(Western Blot using anti-HVEM antibody HMHV-1B18 (MBS488601) Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 () at 0.01ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Western Blot (WB)
(Western Blot using anti-HVEM antibody HMHV-1B18 (MBS488601) Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 () at 0.01ug/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-HVEM antibody HMHV-1B18 (MBS488601) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-HVEM antibody HMHV-1B18 (MBS488601) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-HVEM antibody
Recombinant monoclonal antibody to HVEM. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma HMHV-1B18.
This antibody was raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein.
This antibody was raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein.
Product Categories/Family for anti-HVEM antibody
References
Waka Ishida et al. B and T lymphocyte attenuator regulates the development of antigen-induced experimental conjunctivitis. Graefes Arch Clin Exp Ophthalmol. 2012 Feb;250(2):289-95.
Note on publication: Describe the use of this antibody, together with the anti-BTLA antibody, to investigate the roles that B and T lymphocyte attenuator (BTLA) and herpesvirus entry mediator (HVEM) play in the development of antigen-induced experimental conjunctivitis (EC).
Note on publication: Describe the use of this antibody, together with the anti-BTLA antibody, to investigate the roles that B and T lymphocyte attenuator (BTLA) and herpesvirus entry mediator (HVEM) play in the development of antigen-induced experimental conjunctivitis (EC).
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The HVEM (Catalog #AAA488601) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-HVEM [HMHV-1B18] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's HVEM can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunoprecipitation (IP), Blocking (BL), Functional Assay (FA). This antibody has been used in FACS to demonstrate that lymphatic endothelial cells mediate deletion only via programmed cell death-1 (PD-1) ligand 1 (Tewalt et al 2012) and in Western Blot to study the role of LIGHT in the pathogenesis of hepatitis (Anand et al 2006). This antibody has been also been used in vivo experiments to study the mechanisms by which TNFSF14 functions to promote airway remodelling in asthma (Sibilano et al 2016), to confirm that costimulatory role through HVEM is not necessary for LIGHT-mediated liver inflammation (Anand et at 2006), and to investigate the role that herpesvirus entry mediator plays in the development of experimental conjunctivitis (Ishida et al, 2012). Treatment with this antibody has been observed to diminish plasma levels of antigen-specific IgG1 and IgE antibodies in mouse asthma models (Sibilano et al 2016), to interfere with the LIGHT-HVEM interaction but not interaction between B and T lymphocyte attenuator (BTLA) and HVEM in mouse hepatitis models (Anand et at 2006), and NOT to affect the development of experimental conjunctivitis in either the induction or the effector phase (Ishida et al, 2012). Researchers should empirically determine the suitability of the HVEM for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HVEM, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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