ELISA (EIA)
(Binding curve of anti-PD-1 antibody J43 (MBS488689) to recombinant mouse PD-1 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse PD-1 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488689. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
anti-Mouse PD-1 Monoclonal Antibody | anti-PD-1 antibody
Anti-PD-1 [J43]
Gene Names
Pdcd1; PD-1; Pdc1; Ly101
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Functional Assay, Immunoprecipitation, Immunohistochemistry, Neutralization Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
PD-1; Monoclonal Antibody; Anti-PD-1 [J43]; CD279; PD1; PD 1; Programmed cell death protein 1; Programmed cell death 1; pdcd1; Protein PD-1; mPD-1; anti-PD-1 antibody
Host
Hamster (Armenian)
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Hamster (Armenian) IgG, lambda
Clone Number
J43
Specificity
This antibody is specific for murine PD-1.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-PD-1 antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), depleting, Neutralizing (NEU)
Application Notes
The specificity of this antibody has been confirmed in ELISA analysis, using PD-1 extracellular domain fusion proteins (Agata et al, 1996). Additionally, in flow cytometric analysis, this antibody reacts with PD-1 cDNA-transfected BHK and CHO cells, but not with parental BHK and CHO cells, as well as reacting with lymphocytes from PD-1 cDNA transgenic mice (Agata et al, 1996). This antibody has been used to immunoprecipitate PD-1 from lysates of PD-1 cDNA-transfected BHK and CHO cells (Agata et al, 1996), in flow cytometric quantification of CD4+PD-1+ T cells in murine spleens (Kasagi et al, 2010), and in immunohistochemical analysis of acetone-fixed murine spinal cord and brain tissue sections (Salama et al, 2003). This antibody displays diverse effects in different mouse models of disease. When administered to NZB/W F1 mice, a model of lupus-like nephritis, this antibody has been shown to delay the onset of nephritis and prolong survival, through the depletion of PD-1+ T cells (Kasagi et al, 2010). Antibody-treated NZB/W F1 mice displayed decreased numbers of PD-1+ T cells, and this antibody was confirmed to trigger complement-dependent cytotoxicity in PD-1+ T cells in vitro (Kasagi et al, 2010). Conversely, administration to experimental allergic encephalitis (EAE) and NOD diabetes mice exacerbated disease, through its neutralising activity (Salama et al, 2003; Ansari et al, 2003); this antibody has been shown in vitro to inhibit binding of both PD-L1-Ig and PD-L2-Ig to PD-1 transfected BHK cells (Ansari et al, 2003).
PMID
8671665
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA (EIA)
(Binding curve of anti-PD-1 antibody J43 (MBS488689) to recombinant mouse PD-1 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse PD-1 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488689. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
ELISA (EIA)
(Binding curve of anti-PD-1 antibody J43 (MBS488689) to recombinant mouse PD-1 Fc-Fusion Protein. ELISA Plate coated with recombinant mouse PD-1 Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using MBS488689. For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
Related Product Information for anti-PD-1 antibody
Recombinant monoclonal antibody to PD-1. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma J43.
This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.
This antibody was raised by immunising Armenian hamsters with B12 cells, a PD-1 cDNA transfectant of BHK cells.
Product Categories/Family for anti-PD-1 antibody
References
Agata et al. Expression of the PD-1 antigen on the surface of stimulated mouse T and B lymphocytes. Int Immunol. 1996 May;8(5):765-72.
Note on publication: Describes the original generation of this antibody, and its use in ELISA, flow cytometry and immunoprecipitation analyses.
Note on publication: Describes the original generation of this antibody, and its use in ELISA, flow cytometry and immunoprecipitation analyses.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
31,842 Da
NCBI Official Full Name
Programmed cell death protein 1
NCBI Official Synonym Full Names
programmed cell death 1
NCBI Official Symbol
Pdcd1
NCBI Official Synonym Symbols
PD-1; Pdc1; Ly101
NCBI Protein Information
programmed cell death protein 1; mPD-1; protein PD-1
UniProt Protein Name
Programmed cell death protein 1
UniProt Gene Name
Pdcd1
UniProt Synonym Gene Names
Pd1; Protein PD-1; mPD-1
UniProt Entry Name
PDCD1_MOUSE
Uniprot Description
PDCD1: a single-pass type I membrane protein. A negative regulator of activated T cells, is upregulated in human and mouse 'exhausted' T cells during persistent viral infection. A marker of angioimmunoblastic T-cell lymphoma and germinal center associated T cells.
Protein type: Apoptosis; Membrane protein, integral
Cellular Component: external side of plasma membrane; plasma membrane
Molecular Function: protein binding
Biological Process: negative regulation of apoptosis; negative regulation of tolerance induction; positive regulation of apoptosis
Similar Products
Product Notes
The PD-1 pdcd1 (Catalog #AAA488688) is an Antibody produced from Hamster (Armenian) and is intended for research purposes only. The product is available for immediate purchase. The Anti-PD-1 [J43] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's PD-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Immunoprecipitation (IP), Immunohistochemistry (IHC), depleting, Neutralizing (NEU). The specificity of this antibody has been confirmed in ELISA analysis, using PD-1 extracellular domain fusion proteins (Agata et al, 1996). Additionally, in flow cytometric analysis, this antibody reacts with PD-1 cDNA-transfected BHK and CHO cells, but not with parental BHK and CHO cells, as well as reacting with lymphocytes from PD-1 cDNA transgenic mice (Agata et al, 1996). This antibody has been used to immunoprecipitate PD-1 from lysates of PD-1 cDNA-transfected BHK and CHO cells (Agata et al, 1996), in flow cytometric quantification of CD4+PD-1+ T cells in murine spleens (Kasagi et al, 2010), and in immunohistochemical analysis of acetone-fixed murine spinal cord and brain tissue sections (Salama et al, 2003). This antibody displays diverse effects in different mouse models of disease. When administered to NZB/W F1 mice, a model of lupus-like nephritis, this antibody has been shown to delay the onset of nephritis and prolong survival, through the depletion of PD-1+ T cells (Kasagi et al, 2010). Antibody-treated NZB/W F1 mice displayed decreased numbers of PD-1+ T cells, and this antibody was confirmed to trigger complement-dependent cytotoxicity in PD-1+ T cells in vitro (Kasagi et al, 2010). Conversely, administration to experimental allergic encephalitis (EAE) and NOD diabetes mice exacerbated disease, through its neutralising activity (Salama et al, 2003; Ansari et al, 2003); this antibody has been shown in vitro to inhibit binding of both PD-L1-Ig and PD-L2-Ig to PD-1 transfected BHK cells (Ansari et al, 2003). Researchers should empirically determine the suitability of the PD-1 pdcd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PD-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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