Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD70 antibody TAN1-6 (MBS488618) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of TAN1-6 (MBS488618) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488618, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody)
Rat anti-Mouse CD70 Monoclonal Antibody | anti-CD70 antibody
Anti-CD70 [TAN1-6]
Gene Names
Cd70; Cd27l; CD27LG; Tnfsf7
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunofluorescence
Purity
Purified antibody. Protein A affinity purified
Synonyms
CD70; Monoclonal Antibody; Anti-CD70 [TAN1-6]; TNFSF7; CD27 ligand; CD27-L; Cd27l; CD27LG; CD70 antigen; Ki-24 antigen; Tnfsf7; tumor necrosis factor (ligand) superfamily; member 7; Tumor necrosis factor ligand superfamily member 7; anti-CD70 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a, kappa
Clone Number
TAN1-6
Specificity
This antibody is specific for murine CD70, a 30-33 kDa type II transmembrane glycoprotein. CD70 is a member of the TNF family and is expressed by activated mouse T and B lymphocytes. Interaction of CD70 with CD27, its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CD70 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF), Blocking (BL)
Application Notes
This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004; Taraban et al, 2006).
PMID
15557144
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD70 antibody TAN1-6 (MBS488618) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of TAN1-6 (MBS488618) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488618, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD70 antibody TAN1-6 (MBS488618) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of TAN1-6 (MBS488618) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488618, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody)
Related Product Information for anti-CD70 antibody
Recombinant monoclonal antibody to CD70. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma TAN1-6.
This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein
This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein
Product Categories/Family for anti-CD70 antibody
References
Taraban Vadim et al. Cutting edge: a critical role for CD70 in CD8 T cell priming by CD40-licensed APCs. J Immunol. 2004 Dec 1;173(11):6542-6.
Note on publication: Describe the original generation of this antibody and its subsequent use in FACS and in vivo experiments to demonstrate the critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells.
Note on publication: Describe the original generation of this antibody and its subsequent use in FACS and in vivo experiments to demonstrate the critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
UniProt Accession #
Molecular Weight
21,919 Da
NCBI Official Full Name
CD70 antigen
NCBI Official Synonym Full Names
CD70 antigen
NCBI Official Symbol
Cd70
NCBI Official Synonym Symbols
Cd27l; CD27LG; Tnfsf7
NCBI Protein Information
CD70 antigen; CD27-L; CD27 ligand; Ki-24 antigen; tumor necrosis factor ligand superfamily member 7; tumor necrosis factor (ligand) superfamily, member 7
UniProt Protein Name
CD70 antigen
Protein Family
UniProt Gene Name
Cd70
UniProt Synonym Gene Names
Cd27l; Cd27lg; Tnfsf7; CD27-L
UniProt Entry Name
CD70_MOUSE
Uniprot Description
CD70: Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells. Belongs to the tumor necrosis factor family.
Protein type: Cytokine; Membrane protein, integral
Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane
Molecular Function: protease binding; cytokine activity; tumor necrosis factor receptor binding; receptor binding
Biological Process: cell proliferation; cell-cell signaling; immune response; signal transduction
Research Articles on CD70
Similar Products
Product Notes
The CD70 cd70 (Catalog #AAA488619) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD70 [TAN1-6] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD70 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunofluorescence (IF), Blocking (BL). This antibody has been used in various FACS analyses to reveal a critical role for CD70 in CD8 T cell priming by CD40-licensed antigen-presenting cells (Taraban et al, 2004), to demonstrate the differential requirement for CD70 and CD80/CD86 in dendritic cell-mediated activation of tumor-tolerized CD8 T cells (Bak et al, 2012), to examine how temporal changes in dendritic cell subsets, cross-priming and costimulation via CD70 control CD8(+) T cell responses to influenza (Ballesteros-Tato et al, 2010), and to show that CD70 is downregulated by interaction with CD27 (Kuka et al, 2013). In addition, this antibody has been used in vivo to block the CD70-CD27 interaction as part of the two studies examining how CD70 is critically required for the priming of CD8+ T cells (Taraban et al, 2004; Taraban et al, 2006). Researchers should empirically determine the suitability of the CD70 cd70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD70, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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