ELISA (EIA)
(Binding curve of anti-VISTA antibody 13F3 () to recombinant mouse VISTA Fc-Fusion Protein. ELISA Plate coated with recombinant mouse VISTA Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using . For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
Rabbit anti-Mouse VISTA Monoclonal Antibody | anti-VISTA antibody
Anti-VISTA [13F3]
Gene Names
Vsir; Dies1; PD-1H; VISTA; 4632428N05Rik
Reactivity
Mouse
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
VISTA; Monoclonal Antibody; Anti-VISTA [13F3]; PD-1H; V-domain Ig suppressor of T cell activation; V-type immunoglobulin domain-containing suppressor of T-cell activation; Platelet receptor Gi24; V-set domain-containing immunoregulatory receptor; V-set immunoregulatory receptor; Vsir; Dies1; anti-VISTA antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG, lambda
Clone Number
13F3
Specificity
This antibody is specific for murine VISTA.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-VISTA antibody
ELISA (EIA), Flow Cytometry (FC/FACS), Blocking (BL)
Application Notes
The specificity of this antibody has been confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017; Sergent et al, 2018) and mice infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011). In mice models, this antibody exacerbates experimental autoimmune encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease progression (Sergent et al, 2018). The use of this antibody to achieve a VISTA blockade enhances anti-tumour immune responses in mice models of both transplantable and inducible melanoma, suppressing tumour growth (LeMercier et al, 2014).
Chimeric Use Statement
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
PMID
21383057
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
ELISA (EIA)
(Binding curve of anti-VISTA antibody 13F3 () to recombinant mouse VISTA Fc-Fusion Protein. ELISA Plate coated with recombinant mouse VISTA Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using . For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
ELISA (EIA)
(Binding curve of anti-VISTA antibody 13F3 () to recombinant mouse VISTA Fc-Fusion Protein. ELISA Plate coated with recombinant mouse VISTA Fc-Fusion Protein at a concentration of 5ug/ml. A 3-fold serial dilution from 10,000 ng/ml was performed using . For detection, a 1:4000 dilution of HRP-labelled anti-rabbit antibody was used.)
Related Product Information for anti-VISTA antibody
Recombinant monoclonal antibody to VISTA. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 13F3.
This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein.
This antibody was raised by immunising Armenian hamsters with EL4 cells overexpressing VISTA-RFP, and VISTA-Ig fusion protein.
References
Wang et al. VISTA, a novel mouse Ig superfamily ligand that negatively regulates T cell responses. J Exp Med. 2011 Mar 14;208(3):577-92.
Note on publication: Describes the original generation of this antibody, and its exacerbation of EAE disease progression.
Note on publication: Describes the original generation of this antibody, and its exacerbation of EAE disease progression.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,559 Da
NCBI Official Full Name
platelet receptor Gi24 isoform 2
NCBI Official Synonym Full Names
V-set immunoregulatory receptor
NCBI Official Symbol
Vsir
NCBI Official Synonym Symbols
Dies1; PD-1H; VISTA; 4632428N05Rik
NCBI Protein Information
platelet receptor Gi24
UniProt Protein Name
V-type immunoglobulin domain-containing suppressor of T-cell activation
UniProt Gene Name
Vsir
UniProt Entry Name
VISTA_MOUSE
Uniprot Description
VISTA: May stimulate MMP14-mediated MMP2 activation
Protein type: Membrane protein, integral
Cellular Component: external side of plasma membrane; intercellular junction; plasma membrane
Molecular Function: protein binding
Biological Process: BMP signaling pathway; negative regulation of T cell cytokine production; positive regulation of BMP signaling pathway; stem cell differentiation
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Product Notes
The VISTA vsir (Catalog #AAA488691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-VISTA [13F3] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's VISTA can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS), Blocking (BL). The specificity of this antibody has been confirmed in both ELISA and flow cytometry (Wang et al, 2011). This antibody has been used in flow cytometric analysis to assess VISTA expression in murine lupus models (Ceeraz et al, 2017; Sergent et al, 2018) and mice infected with HSV-1 (Srivastava et al, 2018). This antibody has been shown to block VISTA, and enhances T cell proliferation in vitro (Wang et al, 2011). In mice models, this antibody exacerbates experimental autoimmune encephalomyelitis (Wang et al, 2011) and murine arthritis and lupus disease progression (Sergent et al, 2018). The use of this antibody to achieve a VISTA blockade enhances anti-tumour immune responses in mice models of both transplantable and inducible melanoma, suppressing tumour growth (LeMercier et al, 2014). Researchers should empirically determine the suitability of the VISTA vsir for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "VISTA, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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