Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-Tim-3 antibody 2C12 (MBS488620) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 2C12 (MBS488620) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488620, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Rat anti-Mouse Tim-3 Monoclonal Antibody | anti-Tim-3 antibody
Anti-Tim-3 [2C12]
Gene Names
Havcr2; Tim3; TIM-3; Timd3
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
Purified antibody. Protein A affinity purified
Synonyms
Tim-3; Monoclonal Antibody; Anti-Tim-3 [2C12]; CD366; HAVcr-2; HAVCR2; Hepatitis A virus cellular receptor 2 homolog; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3; TIM3; anti-Tim-3 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG1, kappa
Clone Number
2C12
Specificity
This antibody is specific for murine TIM-3, a Th1-specific cell surface protein. TIM-3 is a type I transmembrane protein and contains an immunoglobulin and a mucin-like domain in its extracellular portion and a tyrosine phosphorylation motif in its cytoplasmic portion. This antibody binds to the BALB/c allele of TIM-3 while reactivity to the C57Bl/6 allele is significantly weaker.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Tim-3 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Blocking (BL), Therapeutics
Application Notes
This antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al, 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al, 2010), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al, 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al, 2005). In addition, employing this anti-TIM-3 antibody as part of the combination immuntherapy (anti-TIM3 + anti-PD-1 +/- anti-CTLA-4) has been shown to be effective against experimental and carcinogen-induced tumors in mice (Sakuishi et al, 2010; Ngiow et al, 2011).
PMID
18006747
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-Tim-3 antibody 2C12 (MBS488620) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 2C12 (MBS488620) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488620, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-Tim-3 antibody 2C12 (MBS488620) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of 2C12 (MBS488620) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488620, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-Tim-3 antibody
Recombinant monoclonal antibody to Tim-3. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 2C12.
This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund's adjuvant.
This antibody was raised by immunising rats with TIM-3:Ig fusion protein emulsified in complete Freund's adjuvant.
Product Categories/Family for anti-Tim-3 antibody
References
Anderson Ana et al. Promotion of tissue inflammation by the immune receptor Tim-3 expressed on innate immune cells. Science. 2007 Nov 16;318(5853):1141-3.
Note on publication: Describe the generation of this antibody and its subsequent use in FACS, immunohistochemistry, immunofluorescence and a murine experimental autoimmune encephalomyelitis (EAE) model to study the effects of TIM-3 triggering in dendritic cells.
Note on publication: Describe the generation of this antibody and its subsequent use in FACS, immunohistochemistry, immunofluorescence and a murine experimental autoimmune encephalomyelitis (EAE) model to study the effects of TIM-3 triggering in dendritic cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
30,934 Da
NCBI Official Full Name
hepatitis A virus cellular receptor 2 homolog
NCBI Official Synonym Full Names
hepatitis A virus cellular receptor 2
NCBI Official Symbol
Havcr2
NCBI Official Synonym Symbols
Tim3; TIM-3; Timd3
NCBI Protein Information
hepatitis A virus cellular receptor 2 homolog; TIMD-3; HAVcr-2; T-cell membrane protein 3; T-cell immunoglobulin and mucin domain containing 3; T-cell immunoglobulin and mucin domain-containing protein 3
UniProt Protein Name
Hepatitis A virus cellular receptor 2 homolog
UniProt Gene Name
Havcr2
UniProt Synonym Gene Names
Tim3; Timd3; HAVcr-2; TIMD-3
UniProt Entry Name
HAVR2_MOUSE
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Product Notes
The Tim-3 havcr2 (Catalog #AAA488621) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Tim-3 [2C12] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Tim-3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Blocking (BL), Therapeutics. This antibody has been used in multiple FACS analyses for diverse immuno-oncological applications, such as to delineate how TIM-3 signaling on cells of the innate immune system critically influences regulation of the adaptive immune response (Frisancho-Kiss et al, 2006), to demonstrate the reversal of T cell exhaustion and restoration of anti-tumor immunity upon co-targeting Tim-3 and PD-1 pathways (Sakuishi et al, 2010), and to examine the therapeutic potential of dendritic cell based vaccines against malignant glioma (Dey et al, 2015). This antibody has also been used in immunohistochemistry to aid the elucidation of the effects of Th2 immune deviation on corneal allograft survival and possible mechanisms of graft rejection (Beauregard et al, 2005). In addition, employing this anti-TIM-3 antibody as part of the combination immuntherapy (anti-TIM3 + anti-PD-1 +/- anti-CTLA-4) has been shown to be effective against experimental and carcinogen-induced tumors in mice (Sakuishi et al, 2010; Ngiow et al, 2011). Researchers should empirically determine the suitability of the Tim-3 havcr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Tim-3, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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