Rabbit anti-Human NUDT8 Polyclonal Antibody | anti-NUDT8 antibody

NUDT8, NT (NUDT8, Nucleoside diphosphate-linked moiety X motif 8, mitochondrial)

Cat. Num. AAA641224

• Gene Names: NUDT8; atnudt8; MQL5.10; MQL5_10; nudix hydrolase homolog 8; NUDT8
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: NUDT8; Polyclonal Antibody; NT (NUDT8; Nucleoside diphosphate-linked moiety X motif 8; mitochondrial); Anti -NUDT8; anti-NUDT8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-NUDT8 antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:50-100; Flow Cytometry: 1:10-50
• Immunogen: NUDT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the N-terminal region of human NUDT8.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(NUDT8 Antibody (N-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the NUDT8 antibody detected the NUDT8 protein (arrow).)

Immunohistochemistry (IHC)

(NUDT8 antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the NUDT8 antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(NUDT8 Antibody (N-term) flow cytometric analysis of MCF-7 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-NUDT8 antibody

Probably mediates the hydrolysis of some nucleoside diphosphate derivatives (By similarity).

Product Categories/Family for anti-NUDT8 antibody

Antibodies; Abs to Mitochondrial Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 18422772
• NCBI GeneID: 834771
• NCBI Accession #: NP_568680.1
• NCBI GenBank Nucleotide #: NM_124095.3
• UniProt Accession #: Q8L7W2; Q8LAJ0; Q9LVT5
• Molecular Weight: 41,314 Da
• NCBI Official Full Name: nudix hydrolase 8
• NCBI Official Symbol: NUDT8
• NCBI Official Synonym Symbols: atnudt8; MQL5.10; MQL5_10; nudix hydrolase homolog 8; NUDT8
• NCBI Protein Information: nudix hydrolase 8
• UniProt Protein Name: Nudix hydrolase 8
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT8
• UniProt Synonym Gene Names: NUDX8; AtNUDT8
• UniProt Entry Name: NUDT8_ARATH

Uniprot Description

Function: Probably mediates the hydrolysis of some nucleoside diphosphate derivatives

By similarity.

Cofactor: Magnesium or manganese

By similarity.

Tissue specificity: Expressed in roots, stems and, at lower level, leaves. Ref.5

Sequence similarities: Belongs to the Nudix hydrolase family.Contains 1 nudix hydrolase domain.

Sequence caution: The sequence BAA97158.1 differs from that shown. Reason: Erroneous gene model prediction.