Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14 (MBS488628) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of RMT2-14 (MBS488628) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488628, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Rat anti-Mouse TIM-2 Monoclonal Antibody | anti-TIM-2 antibody
Anti-TIM-2 [RMT2-14]
Gene Names
Timd2; G9D3; Tim2; TIM-2; C78111; AA816106; AU019358; AU019662
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay, Western Blot, Immunohistochemistry, Functional Assay
Purity
Purified antibody. Protein A affinity purified
Synonyms
TIM-2; Monoclonal Antibody; Anti-TIM-2 [RMT2-14]; TIMD2; T Cell Immunoglobulin Mucin-2; T Cell immunoglobulin and mucin domain protein 2; TIM3; anti-TIM-2 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rat IgG2a, lambda
Clone Number
RMT2-14
Specificity
This antibody is specific for murine TIM-2, a transmembrane protein that is expressed on activated T cells and preferentially on differentiated Th2 cells. A blockade of TIM-2/TIM-2 ligand interaction results in T cell hyperproliferation and Th2 cytokine production. TIM-2 plays a critical role in the development of atopic disease and other Th2-biased immune responses.
Purity/Purification
Purified antibody. Protein A affinity purified
Form/Format
PBS with 0.02% Proclin 300.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-TIM-2 antibody
Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC), Blocking (BL), Functional Assay (FA)
Application Notes
This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011).
PMID
17620455
Preparation and Storage
Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14 (MBS488628) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of RMT2-14 (MBS488628) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488628, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed mouse splenocytes with anti-TIM-2 antibody RMT2-14 (MBS488628) Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix coverslips stained with the chimeric rabbit IgG version of RMT2-14 (MBS488628) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488628, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)
Related Product Information for anti-TIM-2 antibody
Recombinant monoclonal antibody to TIM-2. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma RMT2-14.
This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.
This antibody was raised by immunising Sprague Dawley rats with TIM-2-Ig consisting of the extracellular domain (aa 1-230 of mouse TIM-2) and the Fc portion of mouse IgG2a, emulsified in complete Freund's adjuvant and then fusing lymph node cells from the immunized rats with P3U1 myeloma cells.
Product Categories/Family for anti-TIM-2 antibody
References
Nakae et al. TIM-1 and TIM-3 enhancement of Th2 cytokine production by mast cells. Blood. 2007 Oct 1; 110(7): 2565-2568.
Note on publication: Describe the original generation of this clone RMT2-1, together with another clone (RMT2-14), of this anti-mouse TIM-2 antibody and their subsequent use for evaluation of the expression of TIM family members on mouse mast cells.
Note on publication: Describe the original generation of this clone RMT2-1, together with another clone (RMT2-14), of this anti-mouse TIM-2 antibody and their subsequent use for evaluation of the expression of TIM family members on mouse mast cells.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,504 Da
NCBI Official Full Name
T-cell immunoglobulin and mucin domain-containing protein 2
NCBI Official Synonym Full Names
T cell immunoglobulin and mucin domain containing 2
NCBI Official Symbol
Timd2
NCBI Official Synonym Symbols
G9D3; Tim2; TIM-2; C78111; AA816106; AU019358; AU019662
NCBI Protein Information
T-cell immunoglobulin and mucin domain-containing protein 2; T-cell immunoglobulin and mucin domain containing 2; T-cell immunoglobulin mucin receptor 2; T-cell membrane protein 2; TIMD-2
UniProt Protein Name
T-cell immunoglobulin and mucin domain-containing protein 2
UniProt Gene Name
Timd2
UniProt Synonym Gene Names
Tim2; TIMD-2; TIM-2
UniProt Entry Name
TIMD2_MOUSE
Uniprot Description
TIM-2: Probable receptor for SEMA4A involved in the regulation of T-cell function. The interaction with SEMA4A enhances T-cell activation. Belongs to the immunoglobulin superfamily. TIM family.
Protein type: Membrane protein, integral
Cellular Component: membrane; integral to membrane; plasma membrane
Research Articles on TIM-2
Similar Products
Product Notes
The TIM-2 timd2 (Catalog #AAA488629) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-TIM-2 [RMT2-14] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's TIM-2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC), Blocking (BL), Functional Assay (FA). This antibody has been used in various FACS analyses, for instance, to evaluate the expression of TIM family members on mouse mast cells (Nakae et al, 2007) and to block the H-ferritin binding to TIM-2/L5178Y cells (Kawamoto et al, 2011). This antibody has been used in FACS and immunohistochemistry analyses to show that TIM-2 is expressed on mouse fetal hepatocytes (Watanabe et al, 2007). This antibody has also been used in Western Blots and for blocking experiments in ferritin binding analysis, which suggests that Tim-2 is the H-ferritin receptor on oligodendrocytes (Todorich et al, 2008). In addition, this antibody has been used in collagen-induced arthritis (CIA) models to determine whether TIM-2 contributes to the development of T helper (Th) 1 or Th17 cells and joint inflammation (Kawamoto et al, 2011). Researchers should empirically determine the suitability of the TIM-2 timd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "TIM-2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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