TSPAN8 recombinant protein

TSPAN8 Protein, Human, Recombinant (mFc Tag)

Cat. Num. AAA8121939

• Gene Names: TSPAN8; CO-029; TM4SF3
• Purity: >95% as determined by SDS-PAGE
• Synonyms: TSPAN8; TSPAN8 Protein; Human; Recombinant (mFc Tag); Human TSPAN8/Tetraspanin 8/TM4SF3 Protein (Fc Tag); CO-029 Protein; TM4SF3 Protein; tetraspanin 8; TSPAN8 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Lys110-Asn205

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Asp
• Tag: N-mouse IgG1-Fc
• Protein Construction: A DNA sequence encoding the human TSPAN8 (P19075)(Lys110-Asn205) was expressed with the Fc region of mouse IgG1 at the N-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for TSPAN8 recombinant protein

Background: Tetraspanin 8 (TSPAN8) as an important modulator of melanoma invasiveness, and several of its transcriptional regulators, which affect TSPAN8 expression during melanoma progression toward an invasive stage. p53 as a negative regulator of Tspan8 expression. p53 as a regulator of melanoma invasion and the concept that reactivating p53 could provide a strategy for modulating not only proliferative but also invasive capacity in melanoma treatment. Tetraspanin 8 (TSPAN8) is a tumor-associated antigen implicated in tumor progression and metastasis. TSPAN8 may play an important role in mCRC cell invasion. TSPAN8 was overexpressed in human gastric cancer tissues and gastric cancer cell lines compared with the normal. TSPAN8 overexpression promoted cell proliferation and invasion, while TSPAN8 suppression inhibited cell proliferation and invasion. TSPAN8 could activate the ERK MAPK pathway in gastric cancer cells, and MEK-ERK inhibition reversed the effects of TSPAN8 overexpression on cell proliferation and invasion.

NCBI and Uniprot Product Information

• NCBI GI #: 4759238
• NCBI GeneID: 7103
• NCBI Accession #: NP_004607.1
• NCBI GenBank Nucleotide #: NM_004616.2
• UniProt Accession #: P19075; Q9BS78; B2R7T7
• Molecular Weight: 26,044 Da
• NCBI Official Full Name: tetraspanin-8
• NCBI Official Synonym Full Names: tetraspanin 8
• NCBI Official Symbol: TSPAN8
• NCBI Official Synonym Symbols: CO-029; TM4SF3
• NCBI Protein Information: tetraspanin-8; tspan-8; tumor-associated antigen CO-029; transmembrane 4 superfamily member 3
• UniProt Protein Name: Tetraspanin-8
• UniProt Gene Name: TSPAN8
• UniProt Synonym Gene Names: TM4SF3; Tspan-8
• UniProt Entry Name: TSN8_HUMAN

NCBI Description

The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

TSPAN8: is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. This gene is expressed in different carcinomas. The use of alternate polyadenylation sites has been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q14.1-q21.1

Cellular Component: integral to membrane

Biological Process: negative regulation of blood coagulation