Rat anti-Mouse Tim-4 Monoclonal Antibody | anti-Tim-4 antibody

Anti-Tim-4 [3A1]

Cat. Num. AAA488623

• Gene Names: Timd4; Tim4; TIM-4; B430010N18Rik
• Reactivity: Mouse
• Applications: Flow Cytometry, Functional Assay, Western Blot, ELISA, Immunohistochemistry, Immunofluorescence
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: Tim-4; Monoclonal Antibody; Anti-Tim-4 [3A1]; TIMD4; SMUCKLER; TIM4; Spleen; mucin-containing; knockout of lymphotoxin protein; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIM-4; TIMD-4; anti-Tim-4 antibody

• Host: Rat
• Reactivity: Mouse
• Clonality: Monoclonal
• Isotype: Rat IgG1, kappa
• Clone Number: 3A1
• Specificity: This antibody is specific for murine TIM-4, which unlike other TIM family members, is not expressed on T lymphocytes but rather found on antigen presenting cells such as lymphoid and myeloid DC's and macrophages. TIM-4 has been shown to interact with TIM-1 thereby suggesting a role of TIM-4 in the balance of Th1/Th2 regulation. Additionally TIM-4 has been shown to be a receptor for phosphatidylserine and may play a role in engulfment of apoptotic cells.
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-Tim-4 antibody: Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Blocking (BL), Therapeutics
• Application Notes: This antibody (Clone 3A1), together with two others (clones 5G3 and 3H11), was originally produced from the subcloned hybridomas; all of these 3 produced mAbs bound specifically to murine TIM-4 and not to any other TIM molecule, as demonstrated by both FACS and ELISA analyses (Rodriguez-Manzanet et al, 2008). This antibody has been used in various FACS analyses, for example, to show that TIM-1 and TIM-4 glycoproteins bind phosphatidylserine and mediate uptake of apoptotic cells (Kobayashi et al, 2007), to underscore the requirement of IL-10 from marginal zone precursor B cell subset for costimulatory blockade-induced transplantation tolerance (Lal et al, 2015), and to uncover a mechanism by which the body adapts to fluctuations in erythrocyte integrity (Theurl et al, 2016). This antibody has also been used in immunohistochemistry to discover a new tissue macrophage subset in the thymus that is dependent on transcription factor NR4A1 for development (Tacke et al, 2015), as well as in immunofluorescence to elucidate the mechanisms that control the heterogeneity of tissue macrophages (A-Gonzalez et al, 2013). In addition, the anti-tumour effects of anti-TIM-4 antibodies have been demonstrated in various murine melanoma and colon adenocarcinoma models (Baghdadi et al, Cancer Immunol. Immunother. 2013; Baghdadi et al, Immunity. 2013).
• PMID: 18354194
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of RAW 264.7 cells using anti-Tim-4 antibody (MBS488622) 3A1. Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 cells stained with the chimeric rabbit IgG version of 3A1 (MBS488622) at 10ug/ml followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488622, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (MBS488041) followed by Alexa Fluor 488 secondary antibody.)

Related Product Information for anti-Tim-4 antibody

Recombinant monoclonal antibody to Tim-4. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 3A1.
This antibody was raised by immunising female Lewis rats (Harlan Sprague-Dawley) with Tim-4:Ig fusion protein in complete Freund's adjuvant.

Product Categories/Family for anti-Tim-4 antibody

immunology; costimulation; cell signalling; oncology; immuno-oncology; immune-checkpoint; T cells

References

Rodriguez-Manzanet Roselynn et al. Tim-4 expressed on antigen-presenting cells induces T cell expansion and survival. J Immunol. 2008 Apr 1;180(7):4706-13.
Note on publication: Describe the original generation of this antibody and its subsequent use in FACS and Western Blots for insights into Tim-4 as a costimulatory molecule that promotes T cell expansion and survival by crosslinking Tim-1 on T cells.

NCBI and Uniprot Product Information

• NCBI GI #: 110624776
• NCBI GeneID: 276891
• NCBI Accession #: NP_848874.3
• NCBI GenBank Nucleotide #: NM_178759.4
• UniProt Accession #: Q6U7R4; Q6U7R3; Q8CIC7
• Molecular Weight: 37,548 Da
• NCBI Official Full Name: T-cell immunoglobulin and mucin domain-containing protein 4
• NCBI Official Synonym Full Names: T cell immunoglobulin and mucin domain containing 4
• NCBI Official Symbol: Timd4
• NCBI Official Synonym Symbols: Tim4; TIM-4; B430010N18Rik
• NCBI Protein Information: T-cell immunoglobulin and mucin domain-containing protein 4; T-cell immunoglobulin and mucin domain containing 4; T-cell immunoglobulin mucin receptor 4; T-cell membrane protein 4; TIMD-4; smuckler; spleen, mucin-containing, knockout of lymphotoxin protei
• UniProt Protein Name: T-cell immunoglobulin and mucin domain-containing protein 4
• UniProt Gene Name: Timd4
• UniProt Synonym Gene Names: Tim4; TIMD-4; SMUCKLER; TIM-4
• UniProt Entry Name: TIMD4_MOUSE

Uniprot Description

TIM-4: Phosphatidylserine receptor that enhances the engulfment of apoptotic cells. Involved in regulating T-cell proliferation and lymphotoxin signaling. Ligand for HAVCR1/TIMD1. Belongs to the immunoglobulin superfamily. TIM family.

Protein type: Membrane protein, integral

Cellular Component: membrane; integral to membrane