Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Rabbit anti-Mouse CD86 Monoclonal Antibody | anti-CD86 antibody
Anti-CD86 [PO.3]
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
Immunofluorescence (IF)
(Immunofluorescence staining of fixed RAW264.7 cells with anti-CD86 antibody PO.3 (B467) (MBS488604) Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version of PO.3 () at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom , DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488149) followed by staining with Alexa Fluor 488 secondary antibody.)
This antibody was raised by immunising Sprague Dawley rats with a mouse B cell line, BCL1 and fusing immune splenocytes with P3U1 myeloma cells.
Note on publication: Describe the use of this antibody, together with the anti-CD80 antibody, to confirm the preferential dependence of murine Ab-mediated autoimmunity on the CD86 costimulatory molecule.
NCBI and Uniprot Product Information
Observed: 68 kDa
NCBI Description
This gene encodes a type I membrane protein that is a member of the immunoglobulin superfamily. This protein is expressed by antigen-presenting cells, and it is the ligand for two proteins at the cell surface of T cells, CD28 antigen and cytotoxic T-lymphocyte-associated protein 4. Binding of this protein with CD28 antigen is a costimulatory signal for activation of the T-cell. Binding of this protein with cytotoxic T-lymphocyte-associated protein 4 negatively regulates T-cell activation and diminishes the immune response. Alternative splicing results in several transcript variants encoding different isoforms.[provided by RefSeq, May 2011]
Uniprot Description
CD86: Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as deciding between immunity and anergy that is made by T- cells within 24 hours after activation. Isoform 2 interferes with the formation of CD86 clusters, and thus acts as a negative regulator of T-cell activation. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Immunoglobulin superfamily; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13.33
Cellular Component: cell surface; integral to membrane; plasma membrane
Molecular Function: phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; receptor activity
Biological Process: cell-cell signaling; immune response; phosphoinositide-mediated signaling; positive regulation of cell proliferation; regulation of phosphoinositide 3-kinase cascade; T cell activation; T cell costimulation
Research Articles on CD86
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Product Notes
The CD86 cd86 (Catalog #AAA488604) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD86 [PO.3] reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD86 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC), Blocking (BL), Functional Assay (FA). This antibody has been used in multiple FACS experiments for various immunological applications, such as to demonstrate how the C-type lectin receptor DCIR modulates immunity to tuberculosis (Troegeler et al, 2017), to elucidate the mechanism of Th17 cell differentiation by cholera toxin (Kang et al, 2016), and to evaluate the role of the spleen tyrosine kinase in graft-versus-host disease (Flynn et al, 2015). This antibody has also been used in immunofluorescence to investigate the potential of peritoneal cavity B cells to alleviate murine colitis (Maseda et al, 2013), and in Western Blot to study the effect of interleukin 4-induced gene-1 (IL4I1) overexpression on the expression of M2 markers and M1-associated cytokines (Yue et al, 2015). Furthermore, this antibody has been used, together with the anti-CD80 antibody, in numerous in vivo studies, such as in a NZB/W F1 mouse lupus model to clarify the role of CD80 and CD86 in murine Ab-mediated autoimmunity (Nakajima et al, 1995), in graft bearing recipient BALB/c mice to evaluate the roles of CD80 and CD86 costimulatory molecules in corneal allograft rejection (Kagaya et al, 2002), and in transgenic BALB/c mice to demonstrate the critical contribution of CD80 and CD86 in anterior chamber-associated immune deviation (Tsukahara et al, 2005). Researchers should empirically determine the suitability of the CD86 cd86 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD86, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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