Testing Data
Human GAPDH Assay Kit | GAPDH assay kit
Human GAPDH Inhibitor Screening Kit (Colorimetric)
Reactivity
Human
Synonyms
GAPDH; Human GAPDH Inhibitor Screening Kit (Colorimetric); GAPDH assay kit
Reactivity
Human
Specificity
Potential compounds. Screening; characterizing & studying potential inhibitors of Human GAPDH
Detection Method
Absorbance 450nM
Sample Type
Potential compounds
Features & Benefits
Simple. Sensitive, Quick and easy method for screening potential inhibitors of GAPDH
Preparation and Storage
Store at -20 degree C
Shipping: Gel Pack
Shelf Life: Est.~12 months
Shipping: Gel Pack
Shelf Life: Est.~12 months
Testing Data
Testing Data
Related Product Information for GAPDH assay kit
Glucose 3-Phosphate Dehydrogenase (GAPDH; EC 1.2.1.12) is a cytosolic enzyme, which catalyzes the conversion of Glyceraldehyde 3-Phosphate to 1, 3-Bisphosphoglycerate (BPG) with the reduction of NAD to NADH in the glycolytic pathway. GAPDH is a multifunctional protein involved in many intracellular processes such as apoptosis, membrane trafficking, ion metabolism, nuclear translocation etc. Although GAPDH is considered as housekeeping gene, increased GAPDH activity has been detected in various human cancers. Additionally, knockdown of GAPDH has been shown to decrease cell proliferation, cell migration and metastasis in in vitro experiments. Therefore, targeting this key enzyme is essential for developing novel therapeutics for treating cancer. In our GAPDH Inhibitor Screening Kit, Glyceraldehyde 3-Phosphate is oxidized by GAPDH to generate 1, 3-Bisphosphoglycerate and NADH, which reduces the probe thereby generating a strong absorbance at 450nM. In the presence of GAPDH inhibitors, the reactions are impeded, thus decreasing the rate and/or extent of generation of GAPDH-dependent absorbance at OD 450nM. This kit provides a sensitive, quick, and easy method for screening potential inhibitors of GAPDH. GAPDH Inhibitor Control is included in the kit to compare the efficacy of test inhibitors. The assay is high-throughput adaptable and can be performed in less than 30 min.
Product Categories/Family for GAPDH assay kit
Similar Products
Product Notes
The Human GAPDH (Catalog #AAA8400625) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA8400625 Assay Kit recognizes Human GAPDH. It is sometimes possible for the material contained within the vial of "GAPDH, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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