SDS-Page
(Recombinant JARID1A / KDM5A protein gel. JARID1A / KDM5A protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 196 kDa Purity: >60%)
JARID1A/KDM5A recombinant protein
Recombinant JARID1A/KDM5A protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant JARID1A / KDM5A protein gel. JARID1A / KDM5A protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 196 kDa Purity: >60%)
SDS-Page
(Recombinant JARID1A / KDM5A protein gel. JARID1A / KDM5A protein was run on a 10% SDS-PAGE gel and stained with Coomassie Blue. MW: 196 kDa Purity: >60%)
Testing Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Testing Data
(Recombinant JARID1A / KDM5A protein activity assay. 3 uM H3K4me3 (1-21) peptide was incubated with 100 nM JARID1A / KDM5A in a reaction buffer containing 50 mM HEPES pH 7.5, 0.02% Triton X-100, 100 uM 2-OG, 100 uM ascorbate, 50 uM (NH4)2Fe(SO4)2·6H2O for12 hr. at RT. Then 10 ?l anti-H3K4me2 antibody and SA-XL665 mixture (each 1:100 dilution in HTRF Detection Buffer) was added to each reaction system and incubated for 30 min. All the operations and reactions were performed at room temperature. HTRF assay was used for detection.)
Background: Lysine (K)-specific demethylase 5A (KDM5A), also known as Jumonji, AT rich interactive domain 1A (JARID1A), is a histone demethylase that specifically demethylates lysine 4 of histone H3 (H3K4), thereby playing a central role in defining the histone code. KDM5A demethylates trimethylated and dimethylated, but not monomethylated H3K4. It does not demethylate histone H3K9, H3K27, H3K36, H3K79 or H4K20. KDM5A may function to stimulate transcription mediated by nuclear receptors and may be involved in transcriptional regulation of HOX proteins during cell differentiation. Also, KDM5A may be involved in the transcriptional repression of cytokines such as CXCL12. It has been shown to bind directly with the Retinoblastoma (pRb) protein which regulates cell proliferation. KDM5A also interacts with Rhombotin-2 which functions distinctly in erythropoiesis and in T-cell leukemogenesis.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]
Uniprot Description
Function: Histone demethylase that specifically demethylates 'Lys-4' of histone H3, thereby playing a central role in histone code. Does not demethylate histone H3 'Lys-9', H3 'Lys-27', H3 'Lys-36', H3 'Lys-79' or H4 'Lys-20'. Demethylates trimethylated and dimethylated but not monomethylated H3 'Lys-4'. May stimulate transcription mediated by nuclear receptors. May be involved in transcriptional regulation of Hox proteins during cell differentiation. May participate in transcriptional repression of cytokines such as CXCL12. Ref.8 Ref.9 Ref.11 Ref.12 Ref.13
Cofactor: Binds 1 Fe2+ ion per subunit
By similarity.
Subunit structure: Interacts with SUZ12; the interaction is direct
By similarity. Interacts with the viral protein-binding domain of RB1. Interacts with ESR1, MYC, MYCN and LMO2. Ref.1 Ref.6 Ref.7 Ref.8 Ref.9 Ref.14
Subcellular location: Nucleus › nucleolus. Note: Occupies promoters of genes involved in RNA metabolism and mitochondrial function
By similarity. Ref.6 Ref.9
Domain: The GSGFP motif is required for the interaction with SUZ12
By similarity.
Sequence similarities: Belongs to the JARID1 histone demethylase family.Contains 1 ARID domain.Contains 1 JmjC domain.Contains 1 JmjN domain.Contains 3 PHD-type zinc fingers.
Sequence caution: The sequence AAB28544.1 differs from that shown. Reason: Frameshift at several positions. The sequence BAE06081.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on JARID1A/KDM5A
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Product Notes
The JARID1A/KDM5A kdm5a (Catalog #AAA388146) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "JARID1A/KDM5A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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