Testing Data
(Figure 1. Response of ACTOneTM S1PR2 cell line & parental cell line to S1PACTOneTM S1PR2 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit). After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR2 cell line. EC50 = 152 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Sphingosine-1-Phosphate Receptor 2 (S1PR2) Cell Line | S1PR2 cell line
Sphingosine-1-Phosphate Receptor 2 (S1PR2) ACTOne Stable Cell Line, HEK293
cell based high-throughput screening of human S1PR2 receptor agonists/antagonists
Testing Data
(Figure 1. Response of ACTOneTM S1PR2 cell line & parental cell line to S1PACTOneTM S1PR2 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit). After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR2 cell line. EC50 = 152 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Testing Data
(Figure 1. Response of ACTOneTM S1PR2 cell line & parental cell line to S1PACTOneTM S1PR2 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit). After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR2 cell line. EC50 = 152 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Description: Human S1PR2 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR2. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux (often detectable by calcium-responsive dye) and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescence microplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR2. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the G protein-coupled receptors, as well as the EDG family of proteins. This protein participates in sphingosine 1-phosphate-induced cell proliferation, survival, and transcriptional activation [provided by RefSeq, Jul 2008]
Uniprot Description
EDG5: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Belongs to the G-protein coupled receptor 1 family.
Protein type: Receptor, GPCR; Lipid-binding; GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: integral to membrane; plasma membrane
Molecular Function: integrin binding; G-protein coupled receptor activity; G-protein-coupled receptor binding; lipid binding
Biological Process: G-protein coupled receptor protein signaling pathway; activation of MAPK activity; positive regulation of cell proliferation
Research Articles on S1PR2
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Product Notes
The S1PR2 s1pr2 (Catalog #AAA430591) is a Cell Line produced from HEK-293 CNG cells and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human S1PR2 receptor cell based assay cell based high-throughput screening of human S1PR2 receptor agonists/antagonists. Researchers should empirically determine the suitability of the S1PR2 s1pr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingosine-1-Phosphate Receptor 2 (S1PR2), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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