Testing Data
Muscarinic Acetylcholine Receptor M4 (CHRM4) Cell Line | CHRM4 cell line
Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne Stable Cell Line
Gene Names
CHRM4; HM4; M4R
Synonyms
Muscarinic Acetylcholine Receptor M4 (CHRM4); Muscarinic Acetylcholine Receptor M4 (CHRM4) ACTOne Stable Cell Line; CHRM4 cell line
Sequence Length
479
Application Notes
cAMP dependent human CHRM4 cell based assay.
Cell based high-throughput screening of human CHRM4 agonists/antagonists.
Cell based high-throughput screening of human CHRM4 agonists/antagonists.
Parental Cells
HEK-293 CNG cells
Detection
FlexStation or Microplate reader
Functional Test
This cell line has been tested positive for CHRM4 specific response
Surviving Rate: More than 2.5 million/vial on the second day after thawing
The receptor specific activity is stable for 10 weeks continuous passage.
Surviving Rate: More than 2.5 million/vial on the second day after thawing
The receptor specific activity is stable for 10 weeks continuous passage.
Mycoplasma Contamination Test
This lot of cells has been tested and found to be free of mycoplasma contamination.
Content
Stable cells: 1 mL (1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO)
Parental cells: 1 mL (1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO)
Parental cells: 1 mL (1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO)
Growth Properties
Adherent
Cell Culture Medium
Growth medium: DMEM-10% FBS supplemented with 250 ug/ml G418, 1 ug/ml Puromycin
Freezing medium: 10% DMSO, 90% complete cell culture medium
Freezing medium: 10% DMSO, 90% complete cell culture medium
Shipping Note
Product available only in the USA.
Preparation and Storage
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.
Testing Data
Testing Data
Testing Data #2
Testing Data #2
Related Product Information for CHRM4 cell line
Description: This cell line is a HEK-293-CNG cell line that expresses a recombinant human CHRM4. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
Human CHRM4 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human CHRM4. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux (often detectable by calcium-responsive dye) and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Introduction: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. M4 receptor-deficient mice exhibit increased locomotor simulation in response to D1 agonists, amphetamine and cocaine. Neurotransmission in the striatum influences extrapyramidal motor control, thus alterations in M4 activity may contribute to conditions such as Parkinson's Disease.
Human CHRM4 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human CHRM4. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux (often detectable by calcium-responsive dye) and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Introduction: The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. M4 receptor-deficient mice exhibit increased locomotor simulation in response to D1 agonists, amphetamine and cocaine. Neurotransmission in the striatum influences extrapyramidal motor control, thus alterations in M4 activity may contribute to conditions such as Parkinson's Disease.
Product Categories/Family for CHRM4 cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
53,049 Da
NCBI Official Full Name
muscarinic acetylcholine receptor M4
NCBI Official Synonym Full Names
cholinergic receptor muscarinic 4
NCBI Official Symbol
CHRM4
NCBI Official Synonym Symbols
HM4; M4R
NCBI Protein Information
muscarinic acetylcholine receptor M4
UniProt Protein Name
Muscarinic acetylcholine receptor M4
Protein Family
UniProt Gene Name
CHRM4
NCBI Description
The muscarinic cholinergic receptors belong to a larger family of G protein-coupled receptors. The functional diversity of these receptors is defined by the binding of acetylcholine and includes cellular responses such as adenylate cyclase inhibition, phosphoinositide degeneration, and potassium channel mediation. Muscarinic receptors influence many effects of acetylcholine in the central and peripheral nervous system. The clinical implications of this receptor are unknown; however, mouse studies link its function to adenylyl cyclase inhibition. [provided by RefSeq, Jul 2008]
Uniprot Description
The muscarinic acetylcholine receptor mediates various cellular responses, including inhibition of adenylate cyclase, breakdown of phosphoinositides and modulation of potassium channels through the action of G proteins. Primary transducing effect is inhibition of adenylate cyclase.
Research Articles on CHRM4
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Product Notes
The CHRM4 chrm4 (Catalog #AAA430349) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human CHRM4 cell based assay. Cell based high-throughput screening of human CHRM4 agonists/antagonists. Researchers should empirically determine the suitability of the CHRM4 chrm4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Muscarinic Acetylcholine Receptor M4 (CHRM4), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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