Testing Data
Sphingosine-1-Phosphate Receptor 5 (S1PR5) Cell Line | S1PR5 cell line
Sphingosine-1-Phosphate Receptor 5 (S1PR5) ACTOne Stable Cell Line, HEK293
cell based high-throughput screening of human S1PR5 receptor agonists/antagonists
Testing Data
Testing Data
Testing Data
(Figure 1. Response of ACTOneTM S1PR5 cell line & parental cell line to S1PACTOneTM S1PR5 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit. After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR5 cell line. EC50 = 36 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Testing Data
(Figure 1. Response of ACTOneTM S1PR5 cell line & parental cell line to S1PACTOneTM S1PR5 cells and parental cells were plated overnight in 20 ul culture mediumon a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loading solution(membrane potential dye kit. After 2 hours of incubation at room temperature, two readingswere obtained prior to and 30 min after the addition of S1P. Ratios of the two readings (F/F0) are plotted inthe figure.A. Dose response curve of S1P in ACTOneTM S1PR5 cell line. EC50 = 36 nM in the presence ofPDE inhibitor Ro 20-1724 and ?–adren degree Ceptor agonist isoproterenol.B. Parental cells do not respond to S1P.)
Description: Human S1PR5 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR5. HEK-293 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levelsand consequently result in ion flux (often detectable by calcium-responsive dye) and cell membranedepolarization which can be easily measured with fluorescent Membrane Potential Dye. The assayallows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescencemicroplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR5. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
NCBI and Uniprot Product Information
NCBI Description
The lysosphingolipid sphingosine 1-phosphate (S1P) regulates cell proliferation, apoptosis, motility, and neurite retraction. Its actions may be both intracellular as a second messenger and extracellular as a receptor ligand. S1P and the structurally related lysolipid mediator lysophosphatidic acid (LPA) signal cells through a set of G protein-coupled receptors known as EDG receptors. Some EDG receptors (e.g., EDG1; MIM 601974) are S1P receptors; others (e.g., EDG2; MIM 602282) are LPA receptors.[supplied by OMIM, Mar 2008]
Uniprot Description
EDG8: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. Is coupled to both the G(i/0)alpha and G(12) subclass of heteromeric G-proteins. May play a regulatory role in the transformation of radial glial cells into astrocytes and may affect proliferative activity of these cells. Belongs to the G-protein coupled receptor 1 family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Receptor, GPCR; GPCR, family 1; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 19p13.2
Cellular Component: integral to membrane; plasma membrane
Biological Process: regulation of neuron differentiation
Research Articles on S1PR5
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Product Notes
The S1PR5 s1pr5 (Catalog #AAA430593) is a Cell Line produced from HEK-293 CNG cells and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human S1PR5 receptor cell based assay cell based high-throughput screening of human S1PR5 receptor agonists/antagonists. Researchers should empirically determine the suitability of the S1PR5 s1pr5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingosine-1-Phosphate Receptor 5 (S1PR5), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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