Testing Data
(Figure 1. Response of ACTOne GPBAR1 (TGR5) cell line & parental cell line to LCA. ACTOne GPBAR1 cells and parental cells were plated overnight in 20 ul culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ul/well of 1X Dye-loading solution (Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of LCA. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of LCA in ACTOne TGR5 cell line. EC50 = 3.2 uM in the presence of PDE inhibitor. B. Parental cells do not respond to LCA)
G-protein coupled bile acid receptor 1 (GPBAR1) Cell Line | GPBAR1 cell line
Human G-protein-coupled bile acid receptor 1 (GPBAR1) ACTOne Stable Cell Line
cell-based high-throughput screening of human GPBAR1 agonists/antagonists.
Growth medium (for Parental Cell Line): 90% DMEM, 10% FBS, supplemented supplemented with 250 ug/ml G418
Freezing medium: 10% DMSO, 90% FBS
Testing Data
(Figure 1. Response of ACTOne GPBAR1 (TGR5) cell line & parental cell line to LCA. ACTOne GPBAR1 cells and parental cells were plated overnight in 20 ul culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ul/well of 1X Dye-loading solution (Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of LCA. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of LCA in ACTOne TGR5 cell line. EC50 = 3.2 uM in the presence of PDE inhibitor. B. Parental cells do not respond to LCA)
Testing Data
(Figure 1. Response of ACTOne GPBAR1 (TGR5) cell line & parental cell line to LCA. ACTOne GPBAR1 cells and parental cells were plated overnight in 20 ul culture medium on a BD Biocoat 384 well plate. The next day, cells were dye-loaded with 20 ul/well of 1X Dye-loading solution (Membrane Potential Assay Kit). After 2 hours of incubation at room temperature, two readings were obtained prior to and 30 min after the addition of LCA. Ratios of the two readings (F/F0) are plotted in the figure. A. Dose response curve of LCA in ACTOne TGR5 cell line. EC50 = 3.2 uM in the presence of PDE inhibitor. B. Parental cells do not respond to LCA)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the G protein-coupled receptor (GPCR) superfamily. This enzyme functions as a cell surface receptor for bile acids. Treatment of cells expressing this GPCR with bile acids induces the production of intracellular cAMP, activation of a MAP kinase signaling pathway, and internalization of the receptor. The receptor is implicated in the suppression of macrophage functions and regulation of energy homeostasis by bile acids. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]
Uniprot Description
Receptor for bile acid. Bile acid-binding induces its internalization, activation of extracellular signal-regulated kinase and intracellular cAMP production. May be involved in the suppression of macrophage functions by bile acids.
Research Articles on GPBAR1
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Product Notes
The GPBAR1 gpbar1 (Catalog #AAA430274) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human GPBAR1 cell-based assay cell-based high-throughput screening of human GPBAR1 agonists/antagonists. Researchers should empirically determine the suitability of the GPBAR1 gpbar1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "G-protein coupled bile acid receptor 1 (GPBAR1), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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