Testing Data
5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A) Cell Line | HTR1A cell line
Human 5-hydroxytryptamine (serotonin) receptor 1A (HTR1A) ACTOne Stable Cell Line
cell-based high-throughput screening of human HTR1A agonists/antagonists.
Growth medium (for Parental Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418
Freezing medium: 10% DMSO, 90% FBS
Testing Data
Testing Data
Testing Data
Testing Data
Description: Human HTR1A ACTOne is a HEK293-CNG cell line that expresses recombinant human HTR1A. HEK293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye (Cat# MBS430187). The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a G protein-coupled receptor for 5-hydroxytryptamine (serotonin), and belongs to the 5-hydroxytryptamine receptor subfamily. Serotonin has been implicated in a number of physiologic processes and pathologic conditions. Inactivation of this gene in mice results in behavior consistent with an increased anxiety and stress response. Mutation in the promoter of this gene has been associated with menstrual cycle-dependent periodic fevers. [provided by RefSeq, Jun 2012]
Uniprot Description
G-protein coupled receptor for 5-hydroxytryptamine (serotonin). Also functions as a receptor for various drugs and psychoactive substances. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Beta-arrestin family members inhibit signaling via G proteins and mediate activation of alternative signaling pathways. Signaling inhibits adenylate cyclase activity and activates a phosphatidylinositol-calcium second messenger system that regulates the release of Ca2+ ions from intracellular stores. Plays a role in the regulation of 5-hydroxytryptamine release and in the regulation of dopamine and 5-hydroxytryptamine metabolism. Plays a role in the regulation of dopamine and 5-hydroxytryptamine levels in the brain, and thereby affects neural activity, mood and behavior. Plays a role in the response to anxiogenic stimuli.
Research Articles on HTR1A
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Product Notes
The HTR1A htr1a (Catalog #AAA430276) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human HTR1A cell-based assay cell-based high-throughput screening of human HTR1A agonists/antagonists. Researchers should empirically determine the suitability of the HTR1A htr1a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "5-Hydroxytryptamine (Serotonin) Receptor 1A (HTR1A), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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