Testing Data
Phosphodiesterase 4C (PDE4C) Cell Line | PDE4C cell line
Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell)
Gene Names
PDE4C; DPDE1; PDE21
Synonyms
Phosphodiesterase 4C (PDE4C); Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell (CHO cell); Phosphodiesterase 4C (PDE4C) ACTOne Stable Cell Line; PDE4C cell line
Sequence Length
712
Application Notes
cAMP dependent human PDE4C cell based assay.
Cell based high-throughput screening of human PDE4C agonists/antagonists.
Cell based high-throughput screening of human PDE4C agonists/antagonists.
Parental Cells
CHO-K1-CNG cells
Detection
FlexStation or Microplate reader
Functional Test
This cell line has been tested positive for PDE4C specific response
Surviving Rate: More than 2.5 million/vial on the second day after thawing
the receptor specific activity is stable for 10 weeks continuous passage.
Surviving Rate: More than 2.5 million/vial on the second day after thawing
the receptor specific activity is stable for 10 weeks continuous passage.
Mycoplasma Contamination Test
This lot of cells has been tested and found to be free of mycoplasma contamination.
Content
Stable cells: 1 mL (1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO)
Parental cells: 1 mL (1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO)
Parental cells: 1 mL (1 x 106 cells/mL in 70% DMEM, 20% FBS, 10% DMSO)
Growth Properties
Adherent
Cell Culture Medium
Growth medium (for PDE4C Cells): DMEM-F12 plus 10% FBS supplemented with 250 ug/ml G418, 1 ug/ml Puromycin and 5 ug/ml blasticidin
Growth medium (for Control Cells): DMEM-F12 plus 10% FBS supplemented with 250 ug/ml G418, 1 ug/ml Puromycin and 300 ug/ml hygromycin
Freezing medium: 10% DMSO, 90% complete cell culture medium.
Growth medium (for Control Cells): DMEM-F12 plus 10% FBS supplemented with 250 ug/ml G418, 1 ug/ml Puromycin and 300 ug/ml hygromycin
Freezing medium: 10% DMSO, 90% complete cell culture medium.
High Baseline Fluorescent Signal
Inspect the cell density and morphology under a microscope. High cell density or unhealthy cells can result in high baseline signal.
Do not remove serum-containing medium from cell plates before dye-loading. If a serum-free environment is required, use DPBS buffer containing 0.2 to 0.5% BSA to replace medium.
Do not remove serum-containing medium from cell plates before dye-loading. If a serum-free environment is required, use DPBS buffer containing 0.2 to 0.5% BSA to replace medium.
Response To Agonist Is Lower Than Expected
Check the overall health of cells.
Cell density is too high or too low. Cell number titration may be necessary.
Keep cells growing in medium with proper drug selection.
Check settings of fluorescence readers.
Cell density is too high or too low. Cell number titration may be necessary.
Keep cells growing in medium with proper drug selection.
Check settings of fluorescence readers.
High Well-To-Well Variations
Cells should be evenly distributed among wells. Before plating, microscopically examine the culture to be sure that they have been dissociated into single cells. Leave the cell plates at room temperature for 30 minutes prior to transferring the plates to a cell culture incubator.
Check the liquid handling system for dispensing accuracy. Optimize the settings of liquid handling system so that cell monolayer is not disturbed by dye and compound addition.
Check settings of fluorescence readers.
Check the liquid handling system for dispensing accuracy. Optimize the settings of liquid handling system so that cell monolayer is not disturbed by dye and compound addition.
Check settings of fluorescence readers.
Response From Cells After The Addition Of Buffer Containing Only DMSO
Keep the final DMSO concentration below 1%.
Shipping Note
Product available only in the USA.
Preparation and Storage
Remove the frozen cells from the dry ice packaging and immediately place the cells at a temperature below -130 degree C, preferably in liquid nitrogen vapor, until ready for use.
Low Survival Rate of Cells After Thawing: Cell vials could have thawed accidentally. Store cell vials in liquid nitrogen immediately after receiving and keep frozen at all time.
Leaving the vial at 37 degree C for too long during thawing will lower the survival rate. Place the vial at 37 degree C until cells are just thawed.
Handle the cells gently. Don't tap the vial or pipette the cells too many times before plating the cells.
Replace the medium four hours after thawing or when the cells have settled to remove DMSO.
Slow Growth Rate of Cells: Do not split cells before they have completely recovered from thawing and reach at least 50% confluence.
Do not dilute cells excessively while splitting.
Spit cells before they reach 80 - 90% confluence.
Use Trypsin-EDTA solution to dissociate cells
Cells may not be able to recover to an optimal stage if trypsin- free dissociation buffer is used.
Low Survival Rate of Cells After Thawing: Cell vials could have thawed accidentally. Store cell vials in liquid nitrogen immediately after receiving and keep frozen at all time.
Leaving the vial at 37 degree C for too long during thawing will lower the survival rate. Place the vial at 37 degree C until cells are just thawed.
Handle the cells gently. Don't tap the vial or pipette the cells too many times before plating the cells.
Replace the medium four hours after thawing or when the cells have settled to remove DMSO.
Slow Growth Rate of Cells: Do not split cells before they have completely recovered from thawing and reach at least 50% confluence.
Do not dilute cells excessively while splitting.
Spit cells before they reach 80 - 90% confluence.
Use Trypsin-EDTA solution to dissociate cells
Cells may not be able to recover to an optimal stage if trypsin- free dissociation buffer is used.
Testing Data
Testing Data
Testing Data #2
(Response of ACTOne PDE4C cells & parental cells to Rolipram)
Testing Data #2
(Response of ACTOne PDE4C cells & parental cells to Rolipram)
Related Product Information for PDE4C cell line
Description: This cell line is a CHO-K1-CNG cell line that expresses a recombinant human PDE4C. CHO-K1-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently results in ion flux and cell membrane depolarization.
Human PDE4C ACTOneTM is a CHO-K1 CNG cell line that expresses recombinant human PDE4C. CHO-K1 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Introduction: The PDE4C receptor, also known as the Phosphodiesterase 4C receptor, is a member of the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes.
Human PDE4C ACTOneTM is a CHO-K1 CNG cell line that expresses recombinant human PDE4C. CHO-K1 CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye. The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Introduction: The PDE4C receptor, also known as the Phosphodiesterase 4C receptor, is a member of the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes.
Product Categories/Family for PDE4C cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
67,791 Da
NCBI Official Full Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4C isoform 1
NCBI Official Synonym Full Names
phosphodiesterase 4C
NCBI Official Symbol
PDE4C
NCBI Official Synonym Symbols
DPDE1; PDE21
NCBI Protein Information
cAMP-specific 3',5'-cyclic phosphodiesterase 4C
UniProt Protein Name
cAMP-specific 3',5'-cyclic phosphodiesterase 4C
Protein Family
UniProt Gene Name
PDE4C
UniProt Synonym Gene Names
DPDE1
NCBI Description
The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE4 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Jul 2011]
Uniprot Description
Hydrolyzes the second messenger cAMP, which is a key regulator of many important physiological processes.
Research Articles on PDE4C
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Product Notes
The PDE4C pde4c (Catalog #AAA430350) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human PDE4C cell based assay. Cell based high-throughput screening of human PDE4C agonists/antagonists. Researchers should empirically determine the suitability of the PDE4C pde4c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Phosphodiesterase 4C (PDE4C), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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