Rabbit anti-Mouse Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 Polyclonal Antibody | anti-AIMP1 antibody

Polyclonal Antibody to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1)

Cat. Num. AAA2028408

• Gene Names: Aimp1; p43; Emap2; Scye1; EMAPII; AIMP1/p43; 9830137A06Rik
• Reactivity: Mouse
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
• Purity: Affinity Chromatography
• Synonyms: Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1; Polyclonal Antibody; Polyclonal Antibody to Aminoacyl tRNA Synthetase Complex Interacting Multifunctional Protein 1 (AIMP1); anti-AIMP1 antibody

• Host: Rabbit
• Reactivity: Mouse
• Clonality: Polyclonal
• Specificity: The antibody is a rabbit polyclonal antibody raised against AIMP1. It has beenselected for its ability to recognize AIMP1 in immunohistochemical staining andwestern blotting.
• Purity/Purification: Affinity Chromatography
• Form/Format: Liquid
• Concentration: 500ug/mL (varies by lot)
• Sequence: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- ATNDAVLKR LEQKGAEADQ IIEYLKQQVA LLKEKAILQA TMREEKKLRV ENAKLKKEIE ELKQELILAE IHNGVEQVRV RLSTPLQTNC TASESVVQSP SVATTASPAT KEQIKAGEEK KVKEKTEKKG EKKEKQQSAA ASTDSKPIDA SRLDLRIGCI VTAKKHPDAD SLYVEEVDVG EAAPRTVVSG LVNHVPLEQM QNRMVVLLCN LKPAKMRGVL SQAMVMCASS PEKVEILAPP NGSVPGDRIT FDAFPGEPDK
• Sequence Length: 336

• Applicable Applications for anti-AIMP1 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
• Application Notes: Western blotting: 0.5-2ug/mL; Immunocytochemistry in formalin fixed cells: 5-20ug/mL; Immunohistochemistry in formalin fixed frozen section: 5-20ug/mL; Immunohistochemistry in paraffin section: 5-20ug/mL; Optimal working dilutions must be determined by end user.
• Fragment: AIMP1 (Ala2~Lys310)
• Organism Species: Mus musculus (Mouse)
• Cross Reactivity: Mouse
• Conjugate: No Conjugate
• Immunogen: Recombinant AIMP1 (Ala2~Lys310) expressed in E Coli.
• Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2083245
• Preparation and Storage: Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Western Blot: Sample: Lane1: Mouse Heart Tissue; Lane2: Mouse Liver Tissue; Lane3: Mouse Pancreas Tissue; Lane4: Mouse Stomach Tissue.)

Western Blot (WB)

(Western Blot: Sample: Recombinant AIMP1, Mouse.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Kidney Tissue.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Uterus Tissue)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Mouse Liver Tissue.)

NCBI and Uniprot Product Information

• NCBI GI #: 17379579
• NCBI GeneID: 13722
• UniProt Accession #: P31230; Q60659
• Molecular Weight: 33,997 Da
• NCBI Official Full Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• NCBI Official Synonym Full Names: aminoacyl tRNA synthetase complex-interacting multifunctional protein 1
• NCBI Official Symbol: Aimp1
• NCBI Official Synonym Symbols: p43; Emap2; Scye1; EMAPII; AIMP1/p43; 9830137A06Rik
• NCBI Protein Information: aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• UniProt Protein Name: Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
• Protein Family: Aminoacyl tRNA synthase complex-interacting multifunctional protein
• UniProt Gene Name: Aimp1
• UniProt Synonym Gene Names: Emap2; Scye1; EMAP-II

Uniprot Description

SCYE1: Non-catalytic component of the multisynthase complex. Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase. Binds tRNA. Possesses inflammatory cytokine activity. Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation. Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels. Promotes dermal fibroblast proliferation and wound repair. Regulates KDELR1- mediated retention of HSP90B1/gp96 in the endoplasmic reticulum. Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations. Induces maturation of dendritic cells and monocyte cell adhesion. Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7. Defects in AIMP1 are the cause of leukodystrophy hypomyelinating type 3 (HLD3). A severe autosomal recessive hypomyelinating leukodystrophy characterized by early infantile onset of global developmental delay, lack of development, lack of speech acquisition, and peripheral spasticity associated with decreased myelination in the central nervous system. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Translation; Translation regulation

Chromosomal Location of Human Ortholog: 3|3 G3

Cellular Component: aminoacyl-tRNA synthetase multienzyme complex; cell surface; cytoplasm; cytoplasmic vesicle; cytosol; endoplasmic reticulum; extracellular region; extracellular space; Golgi apparatus; membrane; nucleus

Molecular Function: cytokine activity; GTPase binding; protein homodimerization activity; RNA binding; tRNA binding

Biological Process: angiogenesis; apoptosis; carbohydrate metabolic process; cell adhesion; cell-cell signaling; glucose metabolic process; inflammatory response; leukocyte migration; negative regulation of endothelial cell proliferation; translation; tRNA aminoacylation for protein translation