Testing Data
Delta-type opioid receptor (OPRD1) Cell Line | OPRD1 cell line
Human Delta-type opioid receptor (OPRD1) ACTOne Stable Cell Line
Gene Names
OPRD1; DOP; DOR; DOR1; OPRD
Synonyms
Delta-type opioid receptor (OPRD1); Human Delta-type opioid receptor (OPRD1) ACTOne Stable Cell Line; Opioid Receptor; Delta 1 (OPRD1) ACTOne Stable Cell Line; Delta 1 (OPRD1); OPRD1 cell line
Sequence Length
372
Application Notes
cAMP dependent assay for Gi-coupled human OPRD1
cell-based high-throughput screening of human OPRD1 agonists/antagonists.
cell-based high-throughput screening of human OPRD1 agonists/antagonists.
Cell Culture Medium
Growth medium (for OPRD1 Stable Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418, 1 ug/ml Puromycin
Growth medium (for Parental Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418
Freezing medium: 10% DMSO, 90% FBS
Growth medium (for Parental Cell Line): 90% DMEM, 10% FBS, supplemented with 250 ug/ml G418
Freezing medium: 10% DMSO, 90% FBS
Mycoplasma Contamination Test
This cell line has been tested negative for Mycoplasma sp.
Growth Properties
Adherent
Detection
FlexStation or Microplate reader
Shipping Note
Product available only in the USA.
Testing Data
Testing Data
Testing Data
Testing Data
Related Product Information for OPRD1 cell line
Introduction: OPRD1 is a G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. It inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. It plays a role in the perception of pain and in opiate-mediated analgesia. It also plays a role in developing analgesic tolerance to morphine.
Description: Human OPRD1 ACTOne is a HEK293-CNG cell line that expresses recombinant human HTR1A. HEK293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye (Cat# MBS430187). The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Description: Human OPRD1 ACTOne is a HEK293-CNG cell line that expresses recombinant human HTR1A. HEK293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization which can be easily measured with fluorescent Membrane Potential Dye (Cat# MBS430187). The assay allows both end-point and kinetic measurement of intracellular cAMP changes with a FLIPR, or a fluorescence microplate reader.
Product Categories/Family for OPRD1 cell line
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,369 Da
NCBI Official Full Name
delta-type opioid receptor
NCBI Official Synonym Full Names
opioid receptor delta 1
NCBI Official Symbol
OPRD1
NCBI Official Synonym Symbols
DOP; DOR; DOR1; OPRD
NCBI Protein Information
delta-type opioid receptor
UniProt Protein Name
Delta-type opioid receptor
Protein Family
UniProt Gene Name
OPRD1
UniProt Synonym Gene Names
OPRD; D-OR-1; DOR-1
Uniprot Description
G-protein coupled receptor that functions as receptor for endogenous enkephalins and for a subset of other opioids. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling leads to the inhibition of adenylate cyclase activity. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Plays a role in the perception of pain and in opiate-mediated analgesia. Plays a role in developing analgesic tolerance to morphine.
Research Articles on OPRD1
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Product Notes
The OPRD1 oprd1 (Catalog #AAA430278) is a Cell Line and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent assay for Gi-coupled human OPRD1 cell-based high-throughput screening of human OPRD1 agonists/antagonists. Researchers should empirically determine the suitability of the OPRD1 oprd1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Delta-type opioid receptor (OPRD1), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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