Testing Data
Sphingosine-1-Phosphate Receptor 3 (S1PR3) Cell Line | S1PR3 cell line
Sphingosine-1-Phosphate Receptor 3 (S1PR3) ACTOne Stable Cell Line, HEK293
cell based high-throughput screening of human S1PR3 receptor agonists/antagonists
Testing Data
Testing Data
Testing Data
(Figure 1. Response of ACTOneTM S1PR3 cell lineACTOneTM S1PR3 receptor cells and parental cells were plated overnight in 20 ul culturemedium on a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loadingsolution (EliteTM Non-Wash Calcium Dye). After 1 hour of incubation at 37 degree C, the plate wasread on Hmamastu FDSS 7000 with online addition of the ligand. Fluorescent intensity was used to plot thefigure.A. Dose response curve of S1P in ACTOneTM S1PR3 cell line. EC50 = 20 nM*Parental cells do not respond to S1P (data not shown))
Testing Data
(Figure 1. Response of ACTOneTM S1PR3 cell lineACTOneTM S1PR3 receptor cells and parental cells were plated overnight in 20 ul culturemedium on a 384 well Bi degree Coat plate. The next day, cells were dye-loaded with 20 ul/well of 1x Dye-loadingsolution (EliteTM Non-Wash Calcium Dye). After 1 hour of incubation at 37 degree C, the plate wasread on Hmamastu FDSS 7000 with online addition of the ligand. Fluorescent intensity was used to plot thefigure.A. Dose response curve of S1P in ACTOneTM S1PR3 cell line. EC50 = 20 nM*Parental cells do not respond to S1P (data not shown))
Description: Human S1PR3 ACTOneTM is a HEK-293 CNG cell line that expresses recombinant human S1PR3. HEK-293 CNG cellsexpress a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levelsand consequently result in ion flux (often detectable by calcium-responsive dye) and cell membranedepolarization which can be easily measured with fluorescent Membrane Potential Dye. The assayallows both end-point and kinetic measurement of intracellular cAMP changes with a FDSS, FLIPR, or a fluorescencemicroplate reader.
This cell line is a HEK-293-CNG cell line that expresses a recombinant human S1PR3. HEK-293-CNG cells express a modified CNG (Cyclic Nucleotide Gated) channel that opens in response to elevated intracellular cAMP levels and consequently result in ion flux and cell membrane depolarization.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the EDG family of receptors, which are G protein-coupled receptors. This protein has been identified as a functional receptor for sphingosine 1-phosphate and likely contributes to the regulation of angiogenesis and vascular endothelial cell function. [provided by RefSeq, Jul 2008]
Uniprot Description
S1PR3: Receptor for the lysosphingolipid sphingosine 1- phosphate (S1P). S1P is a bioactive lysophospholipid that elicits diverse physiological effect on most types of cells and tissues. When expressed in rat HTC4 hepatoma cells, is capable of mediating S1P-induced cell proliferation and suppression of apoptosis. Belongs to the G-protein coupled receptor 1 family.
Protein type: Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR; GPCR, family 1
Chromosomal Location of Human Ortholog: 9q22.1-q22.2
Cellular Component: integral to plasma membrane; plasma membrane
Molecular Function: G-protein coupled receptor activity; integrin binding; lipid binding
Biological Process: G-protein coupled receptor protein signaling pathway; anatomical structure morphogenesis; elevation of cytosolic calcium ion concentration; regulation of interleukin-1 beta production; positive regulation of cell proliferation; cytokine production; G-protein signaling, adenylate cyclase inhibiting pathway; inflammatory response
Research Articles on S1PR3
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Product Notes
The S1PR3 s1pr3 (Catalog #AAA430592) is a Cell Line produced from HEK-293 CNG cells and is intended for research purposes only. The product is available for immediate purchase. cAMP dependent human S1PR3 receptor cell based assay cell based high-throughput screening of human S1PR3 receptor agonists/antagonists. Researchers should empirically determine the suitability of the S1PR3 s1pr3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Sphingosine-1-Phosphate Receptor 3 (S1PR3), Cell Line" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
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