Typical Testing Data/Standard Curve (for reference only)
Pyruvate Kinase Assay Kit | AT3G55810 assay kit
Pyruvate Kinase Activity Colorimetric / Fluorometric Assay Kit
Synonyms
Pyruvate Kinase; Pyruvate Kinase Activity Colorimetric / Fluorometric Assay Kit; AT3G55810 assay kit
Sequence Length
492
Samples
Purified/Isolated enzyme, Testing inhibitor panels
Features & Benefits
Simple procedure; takes ~ less than 40 minutes
Fast and convenient
Fast and convenient
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for AT3G55810 assay kit
Product Information: Pyruvate Kinase Activity Assay Kit: Colorimetric & Fluorometric Assay for Measuring Pyruvate Kinase Activity in various Biological Samples such as Blood, Tissues, Cultured cells etc. within 30 min. Convenient, Accurate & Reliable. Detection Limit: 0.1 mU. 100 Assays.
Background: Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. MyBioSource provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at lambda = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The kit detects 0.1 mU/ml pyruvate kinase.
Background: Pyruvate kinase (PK, EC 2.7.1.40) is an enzyme involved in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule of pyruvate and one molecule of ATP. Lack of pyruvate kinase will slow down the process of glycolysis which causes the disease known as pyruvate kinase deficiency. MyBioSource provides a simple, direct and automation-ready procedure for measuring pyruvate kinase activity in various biological samples such as blood, tissues, and culture cells, etc. In the assay, PEP and ADP were catalyzed by PK to generate pyruvate and ATP. The generated pyruvate is oxidized by pyruvate oxidase to produce color (at lambda = 570 nm) and fluorescence (at Ex/Em = 535/587 nm). Since the increase in color or fluorescence intensity is proportional to the increase in pyruvate amount, the PK activity can be accurately measured. The kit detects 0.1 mU/ml pyruvate kinase.
Summary: Detection method- Absorbance (570 nm) or Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The kit detects 0.1 mU/ml pyruvate kinase.
Product Categories/Family for AT3G55810 assay kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
52,908 Da
NCBI Official Full Name
pyruvate kinase
NCBI Official Symbol
AT3G55810
NCBI Protein Information
pyruvate kinase
UniProt Protein Name
Pyruvate kinase
Protein Family
UniProt Gene Name
F1I16_220
UniProt Entry Name
Q9M044_ARATH
Similar Products
Product Notes
The AT3G55810 f1i16_220 (Catalog #AAA841626) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Pyruvate Kinase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
