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Testing Data
Mammalian Glyoxalase I Assay Kit
Glyoxalase I Activity Assay Kit (Colorimetric)
Reactivity
Mammalian
Synonyms
Glyoxalase I; Glyoxalase I Activity Assay Kit (Colorimetric); Glyoxalase I assay kit
Reactivity
Mammalian
Samples
Tissue Homogenates: Liver, Kidney, etc
Cell Lysates:U937, Hep G2 cells, etc
Purified Enzyme
Cell Lysates:U937, Hep G2 cells, etc
Purified Enzyme
Detection Method
Colorimetric: OD 240nm
Features & Benefits
The most sensitive kit in the market to detect Glyoxalase I activity
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of Glyoxalase I
Measurement of Glyoxalase I activity in biological samples
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of Glyoxalase I
Measurement of Glyoxalase I activity in biological samples
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
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Related Product Information for Glyoxalase I assay kit
Glyoxalase I (GloI, lactoylglutathione lyase, EC 4.4.1.5) is a cytosolic enzyme that participates in the Glyoxalase system. The glyoxalase system is a metabolic pathway that detoxifies alpha-ketoaldehydes such as methylglyoxal to D-lactic acid, via the intermediate S-D-lactoyl-glutathione (SLG). Specifically, GloI catalyzes the formation of SLG from methylglyoxal and reduced Glutathione. It is overexpressed in several human cancers such as colon, breast, prostate and melanoma. GloI also enhances resistance to antitumor agent-induced apoptosis in human leukemia cells. In addition, recent findings have linked GloI to numerous anxiety-like behaviors including psychiatric diseases (depression, schizophrenia and autism) and pain. Glyoxalase I Activity Assay Kit utilizes the ability of an active GloI to catalyze the formation of SLG by using two GloI substrates. The formation of the SLG can be tracked using a microplate reader at OD 240 nm. Our assay kit is simple, sensitive and can detect as low as 0.05 mU of Glo I activity in biological samples.
Product Categories/Family for Glyoxalase I assay kit
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Product Notes
The Mammalian Glyoxalase I (Catalog #AAA849741) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849741 Assay Kit recognizes Mammalian Glyoxalase I. It is sometimes possible for the material contained within the vial of "Glyoxalase I, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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