Testing Data
Mammalian Glyoxalase II Assay Kit
Glyoxalase II Activity Kit (Colorimetric)
Reactivity
Mammalian
Synonyms
Glyoxalase II; Glyoxalase II Activity Kit (Colorimetric); Glyoxalase II assay kit
Reactivity
Mammalian
Samples
Tissuehomogenatesand cell lysates: Liver,HepG2 cells,etc.
Whole Blood, Red Blood cells
Purified Enzymeor protein preparations
Whole Blood, Red Blood cells
Purified Enzymeor protein preparations
Detection Method
Colorimetric: OD 450nm
Features & Benefits
The most sensitive kit in the market to detect Glyoxalase II activity
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of Glyoxalase II
Measurement of Glyoxalase II activity in biological samples
Simple procedure; takes ~ 40 minutes
Fast and convenient
Kit contains all necessary reagents for accurate measurement of Glyoxalase II
Measurement of Glyoxalase II activity in biological samples
Preparation and Storage
Store at -20 degree C
Shelf life: ~12 months
Shelf life: ~12 months
Testing Data
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Related Product Information for Glyoxalase II assay kit
Glyoxalase II (GloII, hydroxyacylglutathione hydrolase, EC 3.1.2.6) is one of the two enzymes in the Glyoxalase system that is ubiquitously expressed in mammalian, plants and bacteria. The Glyoxalase system is a biological pathway that detoxifies cells by metabolizing alpha-ketoaldehydes such as methylglyoxal, a cytotoxic byproduct of lipids and glucose metabolism. GloII fulfills the terminal step in the glyoxalase system: It catalyzes the hydrolysis of S-D-lactoyl-glutathione (SLG) to reduced glutathione and D-lactic acid. The alteration of GloII activity in cell growth cycle, cell differentiation, phagocyte activation and diabetes mellitus development of implies this enzyme plays a wide-ranging and important role in cell function and disease progression. Research areas of current interest in Glo-II include diabetes, and cancer therapy. Glyoxalase II Activity Kit utilizes the ability of an active Glo-II to cleave a substrate while producing D-lactate. The produced D-Lactate reacts with the chromophore generating a stable signal that can be easily quantified at 450 nm using a microplate reader. Our assay kit is simple, sensitive and can detect as low as 0.6 mU/ml of Glo-II activity in biological samples
Product Categories/Family for Glyoxalase II assay kit
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Product Notes
The Mammalian Glyoxalase II (Catalog #AAA849682) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA849682 Assay Kit recognizes Mammalian Glyoxalase II. It is sometimes possible for the material contained within the vial of "Glyoxalase II, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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