Rabbit anti-Human DVL3 Polyclonal Antibody | anti-DVL3 antibody

DVL3, CT (DVL3, KIAA0208, Segment polarity protein dishevelled homolog DVL-3, DSH homolog 3) (AP)

Cat. Num. AAA6293917

• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DVL3; Polyclonal Antibody; CT (DVL3; KIAA0208; Segment polarity protein dishevelled homolog DVL-3; DSH homolog 3) (AP); DSH homolog 3; anti-DVL3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-DVL3 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: DVL3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 537-565 amino acids from the C-terminal region of human DVL3.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DVL3 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the DVL3 antibody detected the DVL3 protein (arrow).)

Immunohistochemistry (IHC)

(DVL3 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of DVL3 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of DVL3 Antibody (C-term) with A375 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

Related Product Information for anti-DVL3 antibody

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation.

Product Categories/Family for anti-DVL3 antibody

Antibodies; KIAA; orf

References

Gnad, T., et al. Mol. Cancer 9, 31 (2010): Bouteille, N., et al. Oncogene 28(28):2569-2580(2009). Fukukawa, C., et al. Oncogene 28(8):1110-1120(2009). Wei, Q., et al. Lung Cancer 62(2):181-192(2008). Lee, Y.N., et al. Cell. Signal. 20(2):443-452(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 41406097
• NCBI GeneID: 1857
• NCBI Accession #: NP_004414.3
• NCBI GenBank Nucleotide #: NM_004423.3
• UniProt Accession #: Q92997; O14642; Q13531; Q8N5E9; Q92607; D3DNT0
• Molecular Weight: 78,055 Da
• NCBI Official Full Name: segment polarity protein dishevelled homolog DVL-3
• NCBI Official Synonym Full Names: dishevelled segment polarity protein 3
• NCBI Official Symbol: DVL3
• NCBI Protein Information: segment polarity protein dishevelled homolog DVL-3; DSH homolog 3; dishevelled-3; dishevelled, dsh homolog 3; dishevelled 3 (homologous to Drosophila dsh)
• UniProt Protein Name: Segment polarity protein dishevelled homolog DVL-3
• Protein Family: Segment polarity protein dishevelled
• UniProt Gene Name: DVL3
• UniProt Synonym Gene Names: KIAA0208; Dishevelled-3
• UniProt Entry Name: DVL3_HUMAN

NCBI Description

This gene is a member of a multi-gene family which shares strong similarity with the Drosophila dishevelled gene, dsh. The Drosophila dishevelled gene encodes a cytoplasmic phosphoprotein that regulates cell proliferation. [provided by RefSeq, Jul 2008]

Uniprot Description

DVL3: a protein in the WNT/planar cell polarity (PCP) signaling pathway. Dvl2-deficient mice indicate that Dvl2 is essential for normal cardiac morphogenesis, somite segmentation and neural tube closure. Transduces the Wnt signal by interacting with the cytoplasmic Axin complex. Dvl and Axin each contain a DIX domains, which mediate their dynamic polymerization. The Dvl-Axin interaction is essential for Wnt signaling. Interacts through its PDZ domain with the C-terminal regions of VANGL1 and VANGL2. Interacts with Dixin and Rac. There is functional redundancy between Dvl1 and Dvl2 in some phenotypes.

Protein type: Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: cytosol

Molecular Function: protein binding; protease binding; protein heterodimerization activity; frizzled binding; beta-catenin binding; receptor binding

Biological Process: response to drug; positive regulation of JNK activity; Wnt receptor signaling pathway; Wnt receptor signaling pathway, planar cell polarity pathway; positive regulation of transcription, DNA-dependent; Wnt receptor signaling pathway through beta-catenin; positive regulation of protein amino acid phosphorylation