Delta Opioid Receptor Antibody | anti-OPRD1 antibody

Delta Opioid Receptor 3-17, Control Peptide

Cat. Num. AAA555343

• Gene Names: OPRD1; OPRD
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Synonyms: Delta Opioid Receptor; Delta Opioid Receptor 3-17; Control Peptide; anti-OPRD1 antibody

• Reactivity: Human, Mouse, Rat
• Form/Format: Affinity Purified - Liquid
• Sequence Length: 372

• Applicable Applications for anti-OPRD1 antibody: Western Blot (WB)
• Type Info: Rabbit IgG
• Immunogen: Synthetic peptide comprising residues 3-17 LVPSARAELQSSPLV of the mouse and rat DOR-1 protein.

Testing Data

(Image: Western blot analysis with DOR at a dilution of 1:500. Lane 1: 10 ug of human brain lysate and Lane 2: 10 ug rat brain lysate)

Testing Data

(Images: Distinct distribution patterns of DORs in subsets of DRG neurons of mice. Immunostaining with antibodies against DOR13-17 [A: 1:30,000, antibody 1 (ab #1); DiaSorin and C: antibody 2 (ab #2); Neuromics] shows DORs in small DRG neurons and afferent fibers in spinal laminae I-II. This immunostaining pattern is abolished by the antiserum preabsorption or the deletion of Oprd1 exon 1. Reduction in immunostaining is quantitatively assayed by determining the percentage of positive DRG neurons (B; n = 6) and fluorescence intensity (Ifluo.) in the laminae I-II (D; n = 5). **P < 0.01; ***P < 0.001. (Scale bars: A and C, 40 μm.). DOR labeling (anti-DOR13-17, 1:30,000; DiaSorin) associated with vesicles in peptidergic small DRG neurons (E and F) is absent in Oprd1 exon 1-deleted mice (G). Colocalization of DORs and neuropeptides is shown by correlated peaks of Ifluo. measured along lines. (Scale bar: 8 μm.) (H) Immunostaining with antibodies against DOR12-18 (1:60,000; Alomone) shows the presence of DORs on the cell surface of large DRG neurons of mice. (Scale bar: 25 μm.) This staining pattern is abolished by preabsorption and is absent in Oprd1 exon 1-deleted mice. (Scale bar: 80 μm.) (I) Triple-immunostaining shows that DOR+ large DRG neurons contain neither SP nor CGRP. (Scale bar: 80 μm.). www.pnas.org/cgi/doi/10.1073/pnas.1008382107)

Related Product Information for anti-OPRD1 antibody

G protein-coupled receptors (GPCRs) are the largest family of membrane receptors that activate intracellular signaling cascades and undergo endocytosis, recycling, or degradation upon stimulation. The (mu) m-, delta (d) and kappa (k) opioid receptors are GPCRs of the nervous system, which control pain, stress, and addictive behaviors. The delta opioid receptor (DOR-1) is the stereoselective receptor for enkephalins and inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance.

Product Categories/Family for anti-OPRD1 antibody

Antibodies; Opioid Receptors; Delta Opioid Receptor 3-17

NCBI and Uniprot Product Information

• NCBI GI #: 63477962
• NCBI GeneID: 4985
• NCBI Accession #: NP_000902.3
• NCBI GenBank Nucleotide #: NM_000911.3
• Molecular Weight: 40,369 Da
• NCBI Official Full Name: delta-type opioid receptor
• NCBI Official Synonym Full Names: opioid receptor delta 1
• NCBI Official Symbol: OPRD1
• NCBI Official Synonym Symbols: OPRD
• NCBI Protein Information: delta-type opioid receptor
• UniProt Protein Name: Delta-type opioid receptor
• Protein Family: Delta-type opioid receptor
• UniProt Gene Name: OPRD1
• UniProt Synonym Gene Names: OPRD; D-OR-1; DOR-1
• UniProt Entry Name: OPRD_HUMAN

Uniprot Description

DOR-1: delta opioid receptor, a G-protein coupled receptor. Inhibits neurotransmitter release by reducing calcium ion currents and increasing potassium ion conductance. Highly stereoselective. Receptor for enkephalins.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR

Chromosomal Location of Human Ortholog: 1p36.1-p34.3

Cellular Component: integral to plasma membrane; intrinsic to plasma membrane; neuron projection; plasma membrane

Molecular Function: neuropeptide binding; opioid receptor activity; protein binding

Biological Process: activation of CREB transcription factor; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to cyclic nucleotide second messenger; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); immune response; negative regulation of protein oligomerization; neuropeptide signaling pathway; positive regulation of peptidyl-serine phosphorylation; protein import into nucleus, translocation; regulation of mitochondrial membrane potential; sensory perception of pain; synaptic transmission