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Typical Testing Data/Standard Curve (for reference only)

PicoProbe Glucose-6-Phosphate Assay Kit

PicoProbe Glucose-6-Phosphate Fluorometric Assay Kit

Synonyms
PicoProbe Glucose-6-Phosphate; PicoProbe Glucose-6-Phosphate Fluorometric Assay Kit; PicoProbe Glucose-6-Phosphate assay kit
Ordering
For Research Use Only!
Samples
Cell and Tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, growth medium etc.
Features & Benefits
Simple procedure; takes ~ 35-40 minutes to complete the assay
Fast and convenient
Sensitive, efficient and accurate assay
Preparation and Storage
At -20 degree C
Shelf Life: 12 months

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PicoProbe Glucose-6-Phosphate assay kit
Product Information: PicoProbe Glucose-6-Phosphate (G6P) Assay Kit: Fluorometric Assay to Quantify G6P in a variety of Biological Samples such as Tissue, Cells etc. within 15-20 min. Rapid, Convenient & Sensitive. Detection Range: 10 to 500 pmoles. 100 Assays

Background: Glucose-6-phosphate (G6P) is a key intermediate for glucose transport into cells which then enters either metabolic pathways or storage. G6P can enter the glycolytic pathway, the pentose phosphate shunt or be stored as glycogen or starch. G6P is utilized by its dehydrogenase to generate reducing equivalents in the form of NAD(P)H. This is particularly important in red blood cells where a G6PDH deficiency leads to hemolytic anemia. MyBioSource's glucose-6-phosphate Assay Kit is a simple, sensitive and rapid means of quantifying G6P in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product that converts a nearly colorless probe to an intensely fluorescent product (Ex/Em 535/587 nm). The Glucose-6-phosphate Assay Kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 uM in the original sample assuming a dilution of 5X during processing. The PicoProbe G6P Assay Kit is much more sensitive than the G6P Colorimetric Assay Kit for low G6P samples.

Summary: Detection method- Fluorescence (Ex/Em 535/587 nm)
Species reactivity- Mammalian
Applications- The kit can detect G6P in the range of 10 to 500 pmoles which is equivalent to the range of 1-500 uM in the original sample, in a high-throughput screening (HTS) format.

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Product Notes

The PicoProbe Glucose-6-Phosphate (Catalog #AAA841460) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "PicoProbe Glucose-6-Phosphate, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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