Typical Testing Data/Standard Curve (for reference only)
Glucose-6-Phosphate Dehydrogenase Assay Kit | zwf assay kit
Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit
Synonyms
Glucose-6-Phosphate Dehydrogenase; Glucose-6-Phosphate Dehydrogenase Activity Colorimetric Assay Kit; zwf assay kit
Sequence Length
491
Samples
Cell and tissue culture supernatants, urine, plasma and serum, as well as many other biological fluids, etc.
Features & Benefits
Simple procedure; takes ~ 30-60 minutes
Fast and convenient
Fast and convenient
Preparation and Storage
At -20 degree C
Shelf Life: 12 months
Shelf Life: 12 months
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for zwf assay kit
Product Information: Glucose-6-Phosphate Dehydrogenase (G6PDH) Activity Assay Kit: Colorimetric Assay for Measuring G6PDH Activity in Tissues, Erythrocytes etc. Rapid, Simple & Sensitive. Detection Limit: 0.04 mU. 100 Assays
Background: Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. MyBioSource's glucose-6-phosphate dehydrogenase Assay Kit is a simple, sensitive and rapid assay detects the activity of G6PDH in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450nm. The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.
Summary: Detection method- Absorbance (450 nm)
Application- Measurement of G6PDH activity in various tissues and cells - Evaluation of pentose phosphate pathway
Background: Glucose-6-phosphate dehydrogenase (G6PDH) is a cytosolic enzyme in the pentose phosphate pathway, a metabolic pathway that supplies reducing energy to cells (such as erythrocytes) by maintaining the level of the co-enzyme nicotinamide adenine dinucleotide phosphate (NADPH). The NADPH in turn maintains the level of glutathione in these cells that helps protect the red blood cells against oxidative damage. Of greater quantitative importance is the production of NADPH for tissues actively engaged in biosynthesis of fatty acids and/or isoprenoids, such as the liver, mammary glands, adipose tissue, and the adrenal glands. MyBioSource's glucose-6-phosphate dehydrogenase Assay Kit is a simple, sensitive and rapid assay detects the activity of G6PDH in a variety of samples. In the assay, glucose-6-phosphate is oxidized with the generation of a product which is utilized to convert a nearly colorless probe to an intensely colored product with an absorbance at 450nm. The G6PDH Assay Kit can detect as low as 0.04mU G6PDH per well.
Summary: Detection method- Absorbance (450 nm)
Application- Measurement of G6PDH activity in various tissues and cells - Evaluation of pentose phosphate pathway
Product Categories/Family for zwf assay kit
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
55,704 Da
NCBI Official Full Name
glucose-6-phosphate dehydrogenase
UniProt Protein Name
Glucose-6-phosphate 1-dehydrogenase
Protein Family
UniProt Gene Name
zwf
UniProt Synonym Gene Names
G6PD; EDF
UniProt Entry Name
G6PD_ECOLI
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Product Notes
The Glucose-6-Phosphate Dehydrogenase zwf (Catalog #AAA841876) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Glucose-6-Phosphate Dehydrogenase, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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