Western Blot (WB)
(Detection of Mouse Wnt-11 by Western Blot. Western blot shows lysates of mouse embryo (13 d.p.c.)tissue. PVDF Membrane was probed with 2ug/ml of Rat Mouse Wnt-11 Monoclonal Antibody followed by HRP-conjugated Rat IgG Secondary Antibody. A specific band was detected for Wnt-11 at approximately 50kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Rat anti-Mouse Wnt-11 Monoclonal Antibody | anti-WNT-11 antibody
Wnt-11 (Protein Wnt-11, Wnt11)
Reactivity
Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein A/G affinity chromatography.
Synonyms
Wnt-11; Monoclonal Antibody; Wnt-11 (Protein Wnt-11; Wnt11); Anti -Wnt-11 (Protein Wnt-11; anti-WNT-11 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
12K195
Specificity
Recognizes mouse Wnt-11. Does not react to recombinant human Wnt-2, -7a, -7b, recombinant mouse Wnt-1, -3a, -4, -5a, -5b, -8a, -8b, or -9a.
Purity/Purification
Affinity Purified
Purified by Protein A/G affinity chromatography.
Purified by Protein A/G affinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.
Applicable Applications for anti-WNT-11 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 2ug/ml
Immunohistochemistry: 8-25ug/ml
Dilution: Western Blot: 2ug/ml
Immunohistochemistry: 8-25ug/ml
Immunogen
Recombinant corresponding to aa39-79, aa225-297 from mouse Wnt-11 expressed in E.coli.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Mouse Wnt-11 by Western Blot. Western blot shows lysates of mouse embryo (13 d.p.c.)tissue. PVDF Membrane was probed with 2ug/ml of Rat Mouse Wnt-11 Monoclonal Antibody followed by HRP-conjugated Rat IgG Secondary Antibody. A specific band was detected for Wnt-11 at approximately 50kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Detection of Mouse Wnt-11 by Western Blot. Western blot shows lysates of mouse embryo (13 d.p.c.)tissue. PVDF Membrane was probed with 2ug/ml of Rat Mouse Wnt-11 Monoclonal Antibody followed by HRP-conjugated Rat IgG Secondary Antibody. A specific band was detected for Wnt-11 at approximately 50kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Immunohistochemistry (IHC)
(Wnt-11 in Mouse Embryo. Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Mouse Wnt-11 Monoclonal Antibody at 25ug/ml overnight at 4°C. Tissue was stained using the Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
Immunohistochemistry (IHC)
(Wnt-11 in Mouse Embryo. Wnt-11 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Rat Mouse Wnt-11 Monoclonal Antibody at 25ug/ml overnight at 4°C. Tissue was stained using the Mouse HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm of muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
Related Product Information for anti-WNT-11 antibody
Wnt-11 belongs to the Wnt family of secreted, highly conserved, cysteine-rich signaling glcoproteins that are important in vertebrate development. Mouse Wnt-11a is expressed in early development and is required for cardiogenesis and branching morphogenesis of ureters. Wnt-11 signals by a non-canonical pathway and may repress canonical signaling by other Wnts. Mouse and human Wnt-11 shares 96% identity over the sequences expressed in the immunogen.
Product Categories/Family for anti-WNT-11 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
WNT-11 protein
NCBI Official Symbol
WNT-11
NCBI Protein Information
WNT-11 protein
Similar Products
Product Notes
The WNT-11 (Catalog #AAA6011079) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Wnt-11 (Protein Wnt-11, Wnt11) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt-11 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 2ug/ml Immunohistochemistry: 8-25ug/ml. Researchers should empirically determine the suitability of the WNT-11 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt-11, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
