Western Blot (WB)
(Detection of Human and Mouse EBF-2 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody followed by HRP -conjugated Sheep IgG Secondary Antibody. A specific band was detected for EBF-2 at approximately 65kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Sheep anti-Mouse EBF-2 Polyclonal Antibody | anti-EBF2 antibody
EBF-2 (Transcription Factor COE2, Early B-cell Factor 2, EBF2, Metencephalon-mesencephalon-olfactory Transcription Factor 1, MET-mesencephalon-olfactory TF1, MET-mesencephalon-olfactory Transcription Factor 1, Olf-1/EBF-like 3, O/E-3, OE-3, Coe2, Mmot1)
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Western Blot (WB)
(Detection of Human and Mouse EBF-2 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody followed by HRP -conjugated Sheep IgG Secondary Antibody. A specific band was detected for EBF-2 at approximately 65kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Western Blot (WB)
(Detection of Human and Mouse EBF-2 by Western Blot. Western blot shows lysates of 3T3-L1 mouse embryonic fibroblast adipose-like cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Human/Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody followed by HRP -conjugated Sheep IgG Secondary Antibody. A specific band was detected for EBF-2 at approximately 65kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.)
Immunohistochemistry (IHC)
(EBF-2 in Mouse Embryo. EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody at 10ug/ml overnight at 4°C. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
Immunohistochemistry (IHC)
(EBF-2 in Mouse Embryo. EBF-2 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Sheep Mouse EBF-2 Antigen Affinity-purified Polyclonal Antibody at 10ug/ml overnight at 4°C. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit and counterstained with hematoxylin (blue). Specific staining was localized to developing muscle cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the COE (Collier/Olf/EBF) family of non-basic, helix-loop-helix transcription factors that have a well conserved DNA binding domain. The COE family proteins play an important role in variety of developmental processes. Studies in mouse suggest that this gene may be involved in the differentiation of osteoblasts. [provided by RefSeq, Oct 2011]
Uniprot Description
Function: Transcription factor that, in osteoblasts, activates the decoy receptor for RANKL, TNFRSF11B, which in turn regulates osteoclast differentiation. Acts in synergy with the Wnt-responsive LEF1/CTNNB1 pathway. Recognizes variations of the palindromic sequence 5'-ATTCCCNNGGGAATT-3'
By similarity.
Subunit structure: Forms either a homodimer or a heterodimer with a related family member
By similarity.
Subcellular location: Nucleus
Potential.
Sequence similarities: Belongs to the COE family.Contains 1 IPT/TIG domain.
Sequence caution: The sequence BAB13843.1 differs from that shown. Reason: Erroneous initiation.
Research Articles on EBF2
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Product Notes
The EBF2 ebf2 (Catalog #AAA649769) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The EBF-2 (Transcription Factor COE2, Early B-cell Factor 2, EBF2, Metencephalon-mesencephalon-olfactory Transcription Factor 1, MET-mesencephalon-olfactory TF1, MET-mesencephalon-olfactory Transcription Factor 1, Olf-1/EBF-like 3, O/E-3, OE-3, Coe2, Mmot1) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's EBF-2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml. Researchers should empirically determine the suitability of the EBF2 ebf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "EBF-2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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