Western Blot (WB)
(Detection of Mouse Wnt‑8b by Western Blot.)
Rat anti-Human, Mouse Wnt8b Monoclonal Antibody | anti-WNT8B antibody
Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member)
Gene Names
WNT8B; WNT8B
Reactivity
Human, Mouse
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
Wnt8b; Monoclonal Antibody; Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member); Anti -Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member); anti-WNT8B antibody
Host
Rat
Reactivity
Human, Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
7H99
Specificity
Selected for its ability to detect rmWnt-8b in direct ELISAs and Western Blots. In these formats, this antibody shows no crossreactivity with rhWnt-2, -7a, -7b, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, or -9b.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from a 0.2um filtered solution in PBS with 5% trehalose. Reconstitute with sterile PBS, 40-50% glycerol. If 0.2ml is used, the concentration will be 0.5mg/ml.
Applicable Applications for anti-WNT8B antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit for rmWnt-8b is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. This antibody was used at 5ug/ml to detect Wnt-8b in cell extracts. Western Blot buffers are listed below. Wash Buffer Blocking Buffer Blotting Buffer 25 mM Tris, pH 7.4 5% nonfat dry milk 2% nonfat dry milk, 0.5 M NaCl in Wash Buffer in Wash Buffer 0.05% Tween® 20, Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. The detection limit for rmWnt-8b is approximately 5 ng/well., Optimal dilutions should be determined by each laboratory for each application.
Immunogen
E. coli-derived rmWnt-8b
Hybridoma
Produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a rat immunized with a purified, E.coli-derived, recombinant protein consisting of mouse Wnt-8b amino acid (aa) segments 22-52 and 195-269 (Accession # AAB31816) fused via a gly/ser linker (rmWnt-8b).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Detection of Mouse Wnt‑8b by Western Blot.)
Western Blot (WB)
(Detection of Mouse Wnt‑8b by Western Blot.)
Related Product Information for anti-WNT8B antibody
Mouse Wnt-8b (Wingless related integration site family member 8b) is a 40-50 kD secreted glycoprotein member of the Wnt family of proteins that function as ligands for the Frizzled family of receptors. Mature mouse Wnt-8b is 329 aa in length and contains 24 cysteines plus three potential N-linked glycosylation sites. Little is known about Wnt-8b. It appears to play a role in forebrain development. The mature mouse protein is 100%, 98% and 97% aa identical to mature rat, canine and human Wnt-8b, respectively.
Product Categories/Family for anti-WNT8B antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
38,721 Da
NCBI Official Full Name
WNT8B
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 8B
NCBI Official Symbol
WNT8B
NCBI Official Synonym Symbols
WNT8B
NCBI Protein Information
protein Wnt-8b; OTTHUMP00000020285
UniProt Protein Name
Protein Wnt-8b
Protein Family
UniProt Gene Name
WNT8B
UniProt Entry Name
WNT8B_HUMAN
NCBI Description
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 95%, 86% and 71% amino acid identity to the mouse, zebrafish and Xenopus Wnt8B proteins, respectively. The expression patterns of the human and mouse genes appear identical and are restricted to the developing brain. The chromosomal location of this gene to 10q24 suggests it as a candidate gene for partial epilepsy. [provided by RefSeq]
Uniprot Description
WNT8B: Ligand for members of the frizzled family of seven transmembrane receptors. May play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus. Belongs to the Wnt family.
Protein type: Secreted, signal peptide; Secreted
Chromosomal Location of Human Ortholog: 10q24
Cellular Component: proteinaceous extracellular matrix; extracellular space; extracellular region
Molecular Function: frizzled binding
Biological Process: neuron differentiation; nervous system development; cell fate commitment; Wnt receptor signaling pathway; response to retinoic acid; gastrulation; determination of dorsal identity; signal transduction; response to estradiol stimulus
Research Articles on WNT8B
Similar Products
Product Notes
The WNT8B wnt8b (Catalog #AAA602255) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Wnt8b (Wingless-type MMTV (Mouse Mammary Tumor Virus) Integration Site Family Member) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Wnt8b can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Western Blot: This antibody can be used at 1-2ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. Using a colorimetric detection system, the detection limit for rmWnt-8b is approximately 25 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection will increase sensitivity by 5 to 50 fold. This antibody was used at 5ug/ml to detect Wnt-8b in cell extracts. Western Blot buffers are listed below. Wash Buffer Blocking Buffer Blotting Buffer 25 mM Tris, pH 7.4 5% nonfat dry milk 2% nonfat dry milk, 0.5 M NaCl in Wash Buffer in Wash Buffer 0.05% Tween® 20, Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect human or mouse Wnt-8b. The detection limit for rmWnt-8b is approximately 5 ng/well., Optimal dilutions should be determined by each laboratory for each application. Researchers should empirically determine the suitability of the WNT8B wnt8b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Wnt8b, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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