Western Blot (WB)
(Western blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for Collagen XIII a1 at approximately 85-95kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Sheep anti-Human Collagen XIII A1 Polyclonal Antibody
Collagen XIII A1 (Col13a1, Collagen alpha-1(XIII) Chain)
Reactivity
Human
Applications
Western Blot, Immunohistochemistry
Purity
Affinity PurifiedPurified by immunoaffinity chromatography.
Synonyms
Collagen XIII A1; Polyclonal Antibody; Collagen XIII A1 (Col13a1; Collagen alpha-1(XIII) Chain); Anti -Collagen XIII A1 (Col13a1; anti-Collagen XIII A1 antibody
Host
Sheep
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse Collagen XIII A1. Species sequence homology: Recombinant human COL13A1v4 (35%), rhCOL4A1, rhCOL25A1, rhCOL3A1, and rhCOL1A1 (1%).
Purity/Purification
Affinity Purified
Purified by immunoaffinity chromatography.
Purified by immunoaffinity chromatography.
Form/Format
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Applicable Applications for anti-Collagen XIII A1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Dilution: Western Blot: 1ug/ml
Immunohistochemistry: 5-15ug/ml
Immunogen
Recombinant corresponding to aa107-565 from mouse Collagen XIII A1 expressed in NSO cell line.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Western blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for Collagen XIII a1 at approximately 85-95kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Western Blot (WB)
(Western blot shows lysates of HT1080 human fibrosarcoma cell line. PVDF membrane was probed with 1ug/ml of Sheep Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody followed by HRP-conjugated Sheep IgG Secondary Antibody. A specific band was detected for Collagen XIII a1 at approximately 85-95kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)
Testing Data
(CollagenXIII a1 in Mouse Embryo. Collagen XIII a1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti -Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody at 15ug/ml overnight at 4°C. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit aNone Detected counterstained with hematoxylin (blue). Specific staining was localized to spinal cord.)
Testing Data
(CollagenXIII a1 in Mouse Embryo. Collagen XIII a1 was detected in immersion fixed frozen sections of mouse embryo (13 d.p.c.) using Sheep Anti -Mouse Collagen XIII a1 Antigen Affinity-purified Polyclonal Antibody at 15ug/ml overnight at 4°C. Tissue was stained using the Sheep HRP-DAB Cell & Tissue Staining Kit aNone Detected counterstained with hematoxylin (blue). Specific staining was localized to spinal cord.)
Related Product Information for anti-Collagen XIII A1 antibody
Collagen XIII A1 is an 85-95kD protein in the type 2 transmembrane collagen family. Mature mouse Collagen XIII A1 consists of a 40aa cytoplasmic domain, a 19aa transmembrane segment, and a 692aa extracellular domain (ECD). The ECD contains three collagenous regions separated by shorter non-collagenous regions. Within comparable regions of the ECD, mouse Collagen XIII A1 shares 85% and 88% aa sequence identity with human and rat Collagen XIII A1, respectively. Mouse Collagen XIII A1 is extensively spliced, with some isoforms showing a tissue specific distribution. Collagen XIII A1 is widely expressed during development and in the adult. It localizes to intercellular adherens junctions and cell-matrix focal adhesions. Collagen XIII A1 assembles into disulfide-linked trimers, a process that is enhanced by proline hydroxylation. Trimerization involves triple helix formation within the collagenous domains, although portions of the non-collagenous regions can also form coiled coils. The ECD of trimeric Collagen XIII A1 is an extended rod-like structure with two flexible hinges that correspond to non-collagenous regions. Collagen XIII A1 clusters in cholesterol-rich domains on the plasma membrane, and it can be cleaved from the cell surface or intracellularly by a furin-like protease. Collagen XIII A1 binds the extracellular matrix molecules fibronectin, heparin, integrin A1 nidogen-2, perlecan. The shed ECD retains its ability to bind fibronectin and can interfere with matrix formation.
Product Categories/Family for anti-Collagen XIII A1 antibody
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Product Notes
The Collagen XIII A1 (Catalog #AAA6011508) is an Antibody produced from Sheep and is intended for research purposes only. The product is available for immediate purchase. The Collagen XIII A1 (Col13a1, Collagen alpha-1(XIII) Chain) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Collagen XIII A1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Western Blot: 1ug/ml Immunohistochemistry: 5-15ug/ml. Researchers should empirically determine the suitability of the Collagen XIII A1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Collagen XIII A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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