Mouse anti-Human LRRC4 Monoclonal Antibody | anti-LRRC4 antibody

LRRC4 (Leucine-rich Repeat-containing Protein 4, Brain Tumor-associated Protein BAG, Nasopharyngeal Carcinoma-associated Gene 14 Protein, Netrin-G2 Ligand, NGL-2, BAG, NAG14, UNQ554/PRO1111)

Cat. Num. AAA641757

• Gene Names: LRRC4; NAG14; NGL-2
• Reactivity: Human
• Applications: Western Blot
• Purity: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Synonyms: LRRC4; Monoclonal Antibody; LRRC4 (Leucine-rich Repeat-containing Protein 4; Brain Tumor-associated Protein BAG; Nasopharyngeal Carcinoma-associated Gene 14 Protein; Netrin-G2 Ligand; NGL-2; BAG; NAG14; UNQ554/PRO1111); Anti -LRRC4 (Leucine-rich Repeat-containing Protein 4; anti-LRRC4 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2B
• Clone Number: 12K125
• Specificity: Recognizes human LRRC4. Species Crossreactivity: mouse. Does not react with recombinant human LRRC3, rhLRRC4B, or rhLRRC32.
• Purity/Purification: Affinity Purified; Purified by Protein A/G affinity chromatography.
• Form/Format: Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.5mg/ml.

• Applicable Applications for anti-LRRC4 antibody: Western Blot (WB)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1ug/ml
• Immunogen: Recombinant corresponding to aa39-527 from human LRRC4 expressed in CHO cell line.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Detection of Human and Mouse LRRC4 by Western Blot.|Western blot shows lysates of human brain (hypothalamus) tissue and mouse brain tissue. PVDF membrane was probed with 1ug/ml of Mouse Human LRRC4 Monoclonal Antibody followed by HRP-conjugated Mouse IgG Secondary Antibody. A specific band was detected for LRRC4 at approximately 80kD. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.)

Related Product Information for anti-LRRC4 antibody

LRRC4 also called NGL-2 (netrin-G ligand-2) or NAG14 (nasopharyngeal carcinoma- associated gene 14) is a 55kD type I transmembrane protein that is a member of the NGL family of synaptic LRR adhesion molecules. Human LRRC4 cDNA encodes 653aa that include a 38aa signal sequence, a 489aa extracellular domain (ECD), a 21aa transmembrane domain, and a 105aa cytoplasmic domain. The ECD contains nine LRRs aa74-288, a C2 type Ig like domain aa354-440, and a Thr-rich segment aa455-526. Within the ECD, human LRRC4 shares 98% aa identity with mouse and rat, 99% aa identity with canine and bovine, and 99.6% aa identity with equine LRRC4. It also shares 54-55% aa identity with family members LRRC4C/NGL-1 and LRRC4B/NGL-3, but each recognizes different ligands. LRRC4 is predominantly expressed in the brain on neurons and astrocytes as a ligand for netrin-G2 on the dendritic surface of synaptic neurons. It is proposed to regulate the formation of excitatory synapses via recruitment of PSD-95 to the cytoplasmic domain after aggregation of LRRC4 at the surface. It suppresses proliferation by downregulating cell signaling pathways, resulting in altered expression of cell cycle regulating proteins and delay at the late G1 phase. It is thus considered a tumor suppressor protein and is often downregulated in brain tumors, particularly gliomas. Forced expression of LRRC4 in tumor cells slows proliferation and promotes differentiation. Addition of soluable LRRC4 to cultured neurons reduces excitatory synapse formation.

Product Categories/Family for anti-LRRC4 antibody

Antibodies; Abs to Netrin

NCBI and Uniprot Product Information

• NCBI GI #: 15029530
• NCBI GeneID: 64101
• NCBI Accession #: NP_071426.1
• NCBI GenBank Nucleotide #: NM_022143.4
• UniProt Accession #: Q9HBW1; Q14DU9; Q6ZMI8; Q96A85; A4D0Y9
• Molecular Weight: 72,717 Da
• NCBI Official Full Name: leucine-rich repeat-containing protein 4
• NCBI Official Synonym Full Names: leucine rich repeat containing 4
• NCBI Official Symbol: LRRC4
• NCBI Official Synonym Symbols: NAG14; NGL-2
• NCBI Protein Information: leucine-rich repeat-containing protein 4; netrin-G2 ligand; leucine-rich repeat-containing 4; brain tumor-associated protein BAG; brain tumor associated protein LRRC4; nasopharyngeal carcinoma-associated gene 14 protein
• UniProt Protein Name: Leucine-rich repeat-containing protein 4
• Protein Family: Leucine-rich repeat-containing protein
• UniProt Gene Name: LRRC4
• UniProt Synonym Gene Names: BAG; NGL-2
• UniProt Entry Name: LRRC4_HUMAN

NCBI Description

This gene is significantly downregulated in primary brain tumors. The exact function of the protein encoded by this gene is unknown. [provided by RefSeq, Jul 2008]

Uniprot Description

LRRC4: Synaptic adhesion protein. Regulates the formation of exitatory synapses through the recruitment of pre-and-postsynaptic proteins. Organize the lamina/pathway-specific differentiation of dendrites. Plays a important role for auditory synaptic responses. Involved in the suppression of glioma.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 7q31.3

Cellular Component: postsynaptic membrane; dendritic spine; integral to membrane; excitatory synapse; cell junction

Molecular Function: protein binding

Biological Process: synapse organization and biogenesis; regulation of synapse organization and biogenesis